Chemical investigation of the roots of Ligularia sagitta has resulted in the characterization of six eremophilenolides 6β,8βdimethoxy-1Oβ-hydroxyeremophil-7(11)-en-12,8a-olide (1), 6βan-geloyloxy-1Oβ-hydroxy-8β-methoxyeremophil-7(11)-en-12,8a-olide (2), 6/3-(2'-methylbutanoyloxy)-10β-hydroxy-8β-methoxy-eremophil-7(11)-en-12,8a-olide (3), 6β-angeloyloxy-1Oβ-hydro-xy-8a-methoxyeremophil-7(11)-en-12,8β-olide (4), 6β-(2'me-thylbutanoyloxy)-10β-hydroxy-8a-methoxyeremophil-7(11)-en-12.8β-olide (5) and 8β. lOβ-dihvdroxv-6β-methoxveremoohil-7(ll)-en-12,8a-olide (6), together with one monoterpene (3R,4R,65)-3,6-dihydroxy-l-menthene (7), two triterpenes lupeoi (8) and ursolic acid (9), and β-sitosterol (10). The structures of five new constituents (1-5) were elucidated by spectroscopic methods including 2D-NMR experiments. The compounds 1, 5 and 7 showed antibacterial activity by being assayed against Staphylococcus aureus, Bacillus subtilis and Escherichia coll.