Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp.510，成果详细信息-中国科技论文在线

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Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp.510

管华诗， Zhenqing Zhang ， a Guangli Yu ， a ， * Huashi Guan ， a Xia Zhao ， a Yuguo Dub ， * and Xiaolu Jiangc

Carbohydrate Research 339(2004)1475-1481，-0001，（）：

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摘要/描述

Alginate that was puriﬁed from the fermentation solution of marine bacteria Vibro sp.510 under speciﬁc reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri-and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic speciﬁcity of this alginate lyase to alginate is discussed.

关键词: Alginate lyase ， Guluronic acid ， Mannuronic acid ， Alginate ， Oligosaccharides

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