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期刊论文
Prusian blue modified amerometric FIA biosensor: one-step immunoassay for α-fetoprotein
J.-G. Guan et al/Biosensors and Bioelectronics 19(2004)789-794，-0001，（）：
The aim of this study was to develop a rapid method to measure α-fetoprotein (AFP) in human serum by use of one-step sandwich enyme-linked immunosorbent assay (ELISA)-BASED immunobiosensor with disposable scree-printed carbon electrod technology. Prussian blue (PB) was de[psote disomg cyclic volatmmetry (CV) on the surface of electrode to catlalyze H2O2 from the reaction of gluecose oxidease. It took only about 30min to complete. the whole experimental procedure trough flow injection ajalysis (FIA) A detction range obtained is the range from 5 to 500ng/ml AFP. This deterction ceems to be quick, reliable and practoca.
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