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引用
期刊论文
Cloning and localization of vip3A gene of Bacillus thuringiensis
Biotechnology Letters 26: 1425-1428, 2004.,-0001,():
An insecticidal protein gene, vip3A, was cloned from Bacillus thuringiensis strainWB50. The nucleotide sequenceof 2,460 bp (GenBank acc. No. AY295778) showed 99% homology with the known vip3A genes. Using specificprimers for vip3A gene, PCR was performed to demonstrate that the gene was not located on the bacterial chromosomeand this was confirmed by Southern blotting using an internal fragment (486 bp) from vip3A gene as aprobe. The gene was carried on a plasmid of 31.8 kb.
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