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期刊论文
Expressionandcharacterizationof aiiA gene from Bacillus subtilis BS-1
MicrobiologicalResearch 163(2008)711-716，-0001，（）：
AHL-lactonase (AiiA), ametallo-beta-lactamaseproducedby Bacillus thuringiensis, Bacillus cereus and Bacillus anthracis, specificallyhydrolyzes N-acyl-homoserine lactones (AHLs)secretedbyGram-negativebacteriaandtherebyattenuatesthe symptoms causedbyplantpathogens. Inthisstudy, an aiiA gene wasclonedfrom Bacillus subtilis BS-1 byPCRwithapairofdegenerateprimers. Thededuced250 amino acidsequencecontainedtwosmallconservedregions, 103SHLHFDH109 and 166TPGHTPGH173, whicharecharacteristicofthemetallo-beta-lactamasefamily. Homology comparisonrevealedthatthededucedaminoacidsequencehadahigh degree ofsimilaritywiththoseoftheknownAiiAproteinsinthe B. cereus group. Additionally, the aiiA gene wasexpressedin Escherichia coli BL21 (DE3)pLysSand the expressedAiiAproteincouldattenuatethesoftrotsymptomscausedby Erwinia carotovora var. carotovora.
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