Effects of Astragalus polysaccharides (APS)on the expression of immune response genesin head kidney, gill and spleen of thecommon carp, Cyprinus carpio L.Chuntao Yuana, Xuping Pana, Yi Gonga, Aijun Xia b, Guanghong Wub,
International Immunopharmacology (2008) 8, 51-58，-0001，（）：
For fish immune defences, cytokines and anti-microbial peptides (lysozyme) in circulating systemplay important roles. In the present study, the effects of Astragalus polysaccharides (APS)injection on gene expression of interleukin 1β (IL-1β), tumor necrosis factorα (TNF-α) andlysozyme-C in the head kidney, gill and spleen of common carp were determined using semiquantitativereverse transcription-polymerase chain reaction (RT-PCR). After injection of APS,IL-1β mRNA level increased in a dose-dependent manner in the head kidney, while no significantchanges were found in the gill and spleen. High dose of APS up regulated TNF-α transcription inthe gill and spleen, while TNF-α mRNA level decreased significantly in the head kidney of lowdose of APS. Lysozyme-C mRNA levels were up regulated in the gill of low dose of APS and spleenof middle dose of APS. No effect of the APS on lysozyme-C expression was observed in headkidney. These results constitute a first step toward the understanding of APS effect on cytokinesand immune-related gene expression in different organs of common carp.