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期刊论文
Characterization of Transferrin-Modiˆed Procationic-Liposome Protamine-DNA Complexes
YAKUGAKU ZASSHI 127(3) 533-539 (2007),-0001,():
We developed a novel transferrin modied non-viral gene delivery system, transferrin-modied procationicliposome-protamine-DNA complexes (Tf-PLPD) and investigated its characteristics. Blank procationic liposomes were prepared using the lm dispersion lter method. Protamine was used to condense plasmid DNA to form protamine-DNA complexes and the complexes were further incubated with blank procationic liposomes to form PLPD. Transferrin was adsorbed onto the surface of PLPD via an electrostatic interaction, and thus Tf-PLPD was produced. Characteristics such as stability in rat serum, morphology, average particle size, zeta potential, and transfection e‹ciency in HepG2 cells were further investigated. The results indicated that the procationic liposomes remained stable in rat serum for 24 h. Tf-PLPD protected plasmid DNA from enzymatic degradation even after lyophilization. The size distribution of Tf-PLPD was in the range of 240±12 nm and the zeta potential was-24.10±2.5 mV (n=3), respectively. The transfection e‹ciencies of Tf-PLPD were 24.26±2.6 mU b-galactosidase/mg protein. Lyophilization and the presence of serum did not aŠect the transfectivity of Tf-PLPD and the procationic liposomes also had low cytotoxicity to cells.
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