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期刊论文

Typing Y Chromosome STR Haplotypes Using Redesigned Primers

侯一平Yiping Hou M.D.; Ji Zhang M.D.; Dahong Sun Ph.D.; Yinbi Li M.D.; Jin Wu M.D.; Sizhong Zhang M.D.; and Mechthild Prinz Ph.D.

J Forensic Sci 2002; 47 (1): 215-217,-0001,():

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摘要/描述

A panel of Y-specific STR loci, including DYS19, DYS389, DYS390, DYS391, DYS392, and DYS393 was analyzed using horizontal nondenaturing polyacrylamide gel electrophoresis with a discontinuous buffer system (horizontal disk-PAGE). In order to obtain correct results for the larger DYS389 and DYS392 alleles, it was necessary to design new primers that bind closer to the repeat region and lead to a significant reduction of the amplified fragment size. Using the modified primer sets the horizontal disk-PAGE results were consistent with a nondenaturing approach using fluorescent primers and a 377 automated sequencer. The modified procedure also amplifies the second repeat stretch at the duplicated DYS389 locus as a single fragment, which results in an immediate allele identification. The results indicate that horizontal disk-PAGE with silverstaining is a simple approach to type the recommended Y-specific STR markers.

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