Gene expression in the rat brain during prostaglandin D2 andadenosinergically-induced sleep
JOURNAL OF NEUROCHEMISTRY|2008|105|1480-1498，-0001，（）：
Previous studies have supported the hypothesis that macromolecularsynthesis occurs in the brain during sleep as a responseto prior waking activities and that prostaglandin D2(PGD2) is an endogenous sleep substance whose effects aredependent on adenosine A2a receptor-mediated signaling. Wecompared gene expression in the cerebral cortex, basalforebrain, and hypothalamus during PGD2-induced and adenosinergically-induced sleep to results from our previouslypublished study of recovery sleep (RS) after sleep deprivation(SD). Immediate early gene expression in the cortex duringsleep induced by PGD2- or by the selective adenosine A2aagonist CGS21680 showed limited similarity to that observedduring RS while, in the basal forebrain and hypothalamus,widespread activation of immediate early genes not seenduring RS occurred. In all three brain regions, PGD2 andCGS21680 reduced the expression of arc, a transcript whoseexpression is elevated during SD. Using GeneChips_, themajority of genes induced by either PGD2 or CGS21680 wereinduced by both, suggesting activation of the same pathways.However, gene expression induced in the brain after PGD2 orCGS21680 treatment was distinct from that described duringRS after SD and apparently involves glial cell gene activationand signaling pathways in neural-immune interactions.