Callus were induced from the segments of basal part of leaf blade of rye (Secale cereal L) on MS medium supplemented with 3 mg/L 2, 4-D. Embryogenic calli were obtained when the calli derived from leaf tissues were subcultured on N6 medium plus 500 mg/L yeast cxtract, 1-2 mg/L 2, 4-D and 1-2 mg/L kinetin. Somatic embryos frequently were produced after transfering the cultures onto MS or N6 medium with 5% sucrose without growth regulators. The embryos germinated and developed into normal plantlets on hormone-free medium. The regenerated plants were potted. Histological investigation on callus initiation from leaf tissues was carried out.