We designed an artificial liver support system (ALSS) including plasma exchange, charcoal hemoperfusion, plasma bilirubin absorption, charcoal plasma perfusion, hemofiltration and hemodialysis. We chose different methods or their combinations according to the patients' conditions. We investigated the effect of ALSS in 154 patients with severe hepatitis, 72 patients survived. All data were analyzed by SPSS. The effectiveness of ALSS treatment was compared according to different stages (i.e. early, middle and end stages). After each ALSS treatment, the liver function of these patients was greatly improved, the serum endotoxin and HBV-DNA concentrations were significantly decreased, and the serum concentration of aromatic amino acids (AAA) such as methionine decreased while BCAA/AAA ratio increased. Patients treated with ALSS in the early or middle stages of disease had much higher survival rates than patients in the end stage of disease. Thus, we concluded that ALSS is a reliable therapy to advanced liver diseases and treatment in early or middle stages is appropriate.