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刘畅

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期刊论文

Ophiopogonin D prevents H2O2-induced injury in primary human umbilical vein endothelial cells

刘畅Qian J. Jiang F. Wang B. Yu Y. Zhang X. Yin Z. & Liu C.

J. Ethnopharmacol. (2010), 128: 438-445.,-0001,():

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摘要/描述

Vessel endothelium injury caused by reactive oxygen species (ROS) including H2O2 plays a critical role in the pathogenesis of cardiovascular disorders. Therefore, drug targeting ROS elimination has highly clinical values in cardiovascular therapy. The plant of Radix Ophiopogon japonicus is a traditional Chinese herbal medicine that has been commonly used for prevention and treatment of cardiovascular diseases for a long history. However, the effective component mediating its beneficial effects remains unknown. In the present study, we investigated the action of Ophiopogonin D (OP-D), one of the most bioactive components of Radix Ophiopogon japonicus, in an endothelial injury model induced by H2O2. We found that OP-D inhibited mRNA levels of antioxidant, inflammatory and apoptotic genes in a dose-dependent manner. H2O2-induced lipid peroxidation and protein carbonylation were reduced by OP-D pretreatment. Mitochondrial ROS generation and cell apoptosis were also attenuated in OP-D pretreated cells. In addition, OP-D restored cellular total antioxidative capacity and inhibited the release of inflammatory cytokines. Furthermore, OP-D suppressed the enzymatic activity of Catalase, HO-1, and Caspases. Finally, OP-D blocked activation of NF-κB and ERK signaling cascades. Taken together, our findings provide the first evidence that OP-D plays a protective role as an effective antioxidant in H2O2-induced endothelial injury. Ophiopogonin D can be therefore developed as a novel drug for the therapy of cardiovascular disorders.

【免责声明】以下全部内容由[刘畅]上传于[2012年04月09日 11时26分35秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。

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