Grain size and microstructural features of cast superalloy K4169 were investigated under various melting and casting conditions with and without the addition of grain refiners. It is found that lowering pouring temperature and adding refiners to the melt can lead to grain refinement of γmatrix and improve the proportion of equiaxed grains. At a conventional pouring temperature of 1400 ℃, the average size of equiaxed grains could be refined to the order of ASTM 3.2, the proportion of equiaxed grains at transverse cross-section could be improved from 56% to 99%. The results also indicate that the average length of primary dendrite axis is shortened with the addition of refiners, but the secondary dendrite arm spacing keeps almost unchanged because local solidification time remained constant. In addition, the microsegregation of main elements such as Fe, Cr, Nb, Mo and Ti is alleviated with the decrease in grain size, and the grain morphology have transformed from dendrite in coarse- to granulate in fine-grained castings. At higher melt pouring temperature, the amount of microporosity in samples with the addition of refiners can be greatly reduced. The mechanisms of grain refinement and increase in equiaxed grain proportion were proposed.