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期刊论文

A novel model to determine the dipeptides responsible for optimum temperature in F/10 xylanase

李炜疆Liangwei Liuab Minglei Wanga Weilan Shaoa Weijiang Lia*

Process Biochemistry 40(2005)1389-1394,-0001,():

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摘要/描述

A bioinformatics method was used to analyze the characteristic dipeptides responsible for optimum temperature of xylanase in the F/10 family. It was found that the positive dipeptides are: TD, GH, and WY; and the negative dipeptides are: GA, IA, FH, LH, SR, and NH. The calculated temperature fitted the optimum temperature of the xylanase very well and the maximal and minimal optimum temperatures were calculated as, 150.46 and -29.06℃. The thermostable mechanism was discussed and the result is useful for xylanase engineering for high temperature activity, for it also provided position information for engineering.

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