In a thermal simulation experiment of closed-system gold tube-high pressure vessels under a given pressure (50M Pa) and calculation with the specific Kinetics and GOR2Isotope Kinetics softw are's, kinetic parameters of gas generation and methane carbon isotope from Triassic-Jurassic source rock sin the Kuqa dep ression of the Tarim Basin are obtained. The ranges of activation energies of methane paralyzed from Jurassic coal, Jurassic mudstone and Triassic mudstone in the Kuqa Dep ression are 197～268kJömol, 180～260kJ/mol, and 214～289kJ/mol, respectively. The frequency ratios are 5.265×1013/s, 9. 761×1011/s, and 2. 270×1014/s. Th is suggests that there are different activation energies and frequency ratios for methanes generated from Jurassic coal, coal-associated mudstone and T riassic mudstone in the Kuqa Dep ression, and reflect their differences of hydrocarbon generation behaviors. The kinetic parameters of methane carbon isotope are also different in Jurassic coal, Jurassic mudstone and Triassic mudstone. Their average activation energies were 228kJ/mol, 205kJ/mol, and 231kJ/mol, respectively. On the basis of the above research, combined with the geological background, the origin of natural gas in the Yinan-gas pool was further discussed using these kinetic parameters, and its gas accumulation model is thus established. The natural gas of the Yinan 2 gas poolwas primarily derived from Jurassic coal2bearing source rock s at the center of the Yangxia Sag and generally belong to the staged accumulative gas. The Yinan 2 gasw asmainly gathered at 5～0Ma with a Ro range of 1.25%～1. 95% and lost about 25%～ 30%.