-
30浏览
-
0点赞
-
0收藏
-
0分享
-
74下载
-
0评论
-
引用
期刊论文
A novel method for screening species-speci
Nucleic Acids Research, 2002, Vol. 30, No.12 00,-0001,():
We report a method called SSH array which combines the suppression subtraction hybridization (SSH) and DNA array techniques to find speciesspecific DNA probes from genomic DNA (gDNA) for species identification. The method first obtains the differential gDNA fragments between two species by SSH and then hybridizes the differential gDNA fragments with arrays made of multiple whole genomes from several species to screen the unique gDNA fragments for one species. The screened unique gDNA fragments can be used as speciesspecific probes to differentiate the species they represent from all other species. We used five species of the genus Dendrobrium, D.aurantiacum Kerr, D.of ficinale Kimura et Migo, D.nobile Lindl., D.chrysotoxum Lindl. and D.fimbriatum Hook., as experimental materials to study the feasibility of the method. The results showed that the method could ef ficiently obtain different species-specific probes for each of the five species.
【免责声明】以下全部内容由[陆祖宏]上传于[2005年03月04日 22时45分08秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。
本学者其他成果
同领域成果