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期刊论文

Characterization of a calmodulin-regulated Ca2+-dependent-protein-kinaserelated protein kinase, AtCRK1, from Arabidopsis

吕应堂Ying WANG Shuping LIANG Qi-Guang XIE and Ying-Tang LU

Biochem. J. (2004) 383, 73-81 (Printed in Great Britain),-0001,():

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摘要/描述

An AtCRK1 [Arabidopsis thaliana CDPK (Ca2+-dependent protein kinase)-related protein kinase 1] has been characterized molecularly and biochemically. AtCRK1 contains the kinase catalytic domain and a CaM (calmodulin)-binding site. Our results demonstrated thatAtCRK1couldbindCaMinaCa2+-dependent manner. This kinase phosphorylated itself and substrates such as histone IIIS and syntide-2 in a Ca2+-independent manner and the activitywas stimulated by several CaM isoforms through its CaMbinding domain. This domain was localized within a stretch of 39 amino acid residues at positions from 403 to 441 with Kd=67 nM for CaM binding. However, the stimulation amplification of the kinase activity of AtCRK1 by different CaM isoforms was similar.

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