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Centrifugal force stretcher a new of in vitro mechanical cell stimulator
Acta Physiologica Sinica, June 25, 2004, 56 (3): 419-423,-0001,():
A number of mechanical cell stimulators have been used to study the effect of mechanical stmulation on cells in vitro. But the efficiencey of these devices is not fully desirable. We recently developed a new device for mechaical cell stimulation, the centrifugal force stretcher, and compared its efficacy with that of the traditional Flexercell Strain Unit. When the mechanical stretcher circumrotates with certain speed, cardiac myocytes attached on the plate are stretched and elongated by centrifugal force. Neonatal rat cardiac myocytes were isolated by enzymatic dissociation from the hearts of 3~5d old Sprague Dawley rats, and were mechanically stimulated by traditional 20% strech and 180r/min centrifugal force for 12 and 24h. The effects of mechanical stimulation on the hypertrophic response of neonatal rat cardiac myocytes and production of angiotensin Ⅱ(Ang Ⅱ) were examined. Compared with the non-stretch group. the radioactivity of 3H-leucine incororated into the stretch-stimulated cardiac myocytes in the centrifugal force stretch group was significantly higher[(1295. 17±51.19)vs(1122.67±51.63) in 12h; (1447.5±35.96)vs(1210.67±90.92)in 24h, P<0.05].Ang Ⅱ was also dramatically increased by 128% in 12h (P<0.05) and 139% in 24h (P<0.01). After the myocytes was stretched for 24h, the LDH level in the mediun in the Flexercell Strain Unit group was significantly higher than that in the centrifugal force group [(14.5±8.7) U/L vs (7.8±4.3)U/L, and Ang Ⅱ secretion of the cardiac myocytes, and the damage to the cells bronght by this stimulator is relatively slighter in comparison with the Flexercell Strain Unit.
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