Objective To investigate the effect of propofol on hippoeampal Bel-2 protein expression after forebrain isehemia/repeffusion (I/R) in rats. Methods forty-two male Wistar rats weighting 250g～300g were randomly divided into three groups of fourteen animals: control group (C)in which sham operation was performed; I/R group (Ⅰ) in which bilateral common carotid arteries were clamped for 10 mins combined with hypotension(MAP: 35mm Hg～45mmHg)induced by exsanguinations, then 1ml/kg saline was injected into the lateral cerebral ventricle using mierosyringe; propofol group (P)in which 1mg/kg propofol was injected into the lateral cerebral ventricle using mierosyringe after I/R. The end of 24 h reperfusion the animals were decapitated, then coronal tissue sections and hippoeampal tissue were obtained for detection of Bcl-2 protein expression by immuno-histoehemistry technique(n=8) and bel-2 mRNA expression by RT-PCR technique(n=6). Results The expression of Bel-2 protein in group P increased significantly compared with that in group C and Ⅰ (P<0.05). The bcl-2 mRNA expression in group P was enhanced compared with that in group C and i (P<0.05). Conclusion Propofol can enhance the Bel-2 protein expression and increase the bcl-2 mRNA expression after forebrain isehemia-reperfusion in rats.