A bench consisting of a pulse tube refrigerator driven by a standing-wave thermoacoustic prime mover has been set up to study the relationship among stack, regenerator and working fluids. The stack of the thermoacoustic prime mover is packed with dense-mesh wire screens because of their low cost and easy manufacture. The effect of the packing factor in the stack on onset temperature, refrigeration temperature and input power is explored. The optimum packing factor of 1.15 pieces per millimeter has been found experimentally, which supplies an empirical value to satisfy a compromise for enhancing thermoacoustic effect, decreasing heat conduction and fluid-friction losses along the stack. The pulse tube cooler driven by the thermoacoustic prime mover is able to obtain refrigeration temperatures as low as 138 and 196K with helium and nitrogen, respectively. Copyright