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期刊论文

SpeciWc antihepatocellular carcinoma T cells generated by dendritic cells pulsed with hepatocellular carcinoma cell line HepG2 total RNA

任军Liwang Zhanga Hongmei Zhanga Wenchao Liua Hui Wangb Jun Jiac Xiaoming Sia Jun Rena*

Cellular Immunology 238(2005)61-66,-0001,():

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摘要/描述

Dendritic cell (DC) vaccination with the use of total tumor RNA provides the potential to generate a polyclonal immune response to multiple known and unknown tumor antigens without HLA restriction. Our study evaluated this approach as potential immunotherapy for patients with hepatocellular carcinoma (HCC). Immature DCs generated from peripheral blood mononuclear cells of patients with HCC were transfected with HepG2-GFP (HepG2 cells transfected stably with plasmid pEGFP-C3) cells total RNA. Transfected, matured DCs were used to stimulate autologous T cells. Results revealed that DCs transfected with HepG2-GFP cells total RNA expressed EGFP when observed by Xow cytometry. Compared with those before transfection, the expressions of membrane molecules were increased dramatically, and interleukin-12p70 release in the supernatant was elevated signiWcantly. SpeciWc T cells generated by DCs transfected with HepG2-GFP total RNA recognized HLA-matched HepG2 cell lines speciWcally. These Wndings indicate that these RNA-transfected DCs successfully generate speciWc T cells that speciWcally recognize HCC cells. Total tumor RNA-pulsed DCs may have potential as an adjuvant immunotherapy for patients with HCC.

版权说明:以下全部内容由任军上传于   2006年07月27日 23时53分36秒,版权归本人所有。

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