您当前所在位置: 首页 > 学者

尚忠林

  • 12浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 192下载

  • 0评论

  • 引用

期刊论文

Blackwell Publishing Ltd Heterotrimeric G-protein participation in Arabidopsis pollen germination through modulation of a plasmamembrane hyperpolarization-activated Ca2+-permeable channel

尚忠林Yansheng Wu Xiaodong Xu Sujuan Li Ting Liu Ligeng Ma and Zhonglin Shang

New Phytologist (2007) 176: 550-559,-0001,():

URL:

摘要/描述

The role of heterotrimeric G proteins in pollen germination and tube growth was investigated using Arabidopsis thaliana plants in which the gene (GPA) encoding the G-protein α subunit (G α) was null or overexpressed. Pollen germination, free cytosolic calcium concentration ([Ca2+ ] cyt) and Ca2+ channel activity in the plasma membrane (PM) of pollen cells were investigated. Results showed that, compared with pollen grains of the wild type (ecotype Wassilewskija, ws), in vitro germinated pollen of G α null mutants (gpa1-1 and gpa1-2) had lower germination percentages and shorter pollen tubes, while pollen from G α overexpression lines (wG α and cG α) had higher germination percentages and longer pollen tubes. Compared with ws pollen cells, [Ca2+] cyt was lower in gpa1-1 and gpa1-2 and higher in wG α and cG α. In whole-cell patch clamp recordings, a hyperpolarization-activated Ca2+-permeable conductance was identified in the PM of pollen protoplasts. The conductance was suppressed by trivalent cations but insensitive to organic blockers; its permeability to divalent cations was Ba2+>Ca2+>Mg2+>Sr2+>Mn2+. The activity of the Ca2+-permeable channel conductance was down-regulated in pollen protoplasts of gpa1-1 and gpa1-2, and up-regulated in wG α and cG α. The results suggest that G α may participate in pollen germination through modulation of the hyperpolarization-activated Ca2+ channel in the PM of pollen cells.

【免责声明】以下全部内容由[尚忠林]上传于[2010年12月15日 10时53分26秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。

我要评论

全部评论 0

本学者其他成果

    同领域成果