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期刊论文
Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004
Acta Cryst. (2004). D60, 1292-1294，-0001，（）：
The adrenal gland protein AD-004 was identified in the human adrenal gland. Full-length AD-004 contains 172 amino acids, with a predicted molecular weight of about 20kDa. In attempts to crystallize human AD-004, the gene was subcloned into a modified pET vector, pET21-DEST, with an N-terminal His5 tag using the Gateway cloning system, followed by protein expression in Escherichia coli strain BL21(DE3). The protein was purified in two steps to near-homogeneity and was crystallized. The crystals belong to space group P61 or P65, with unit-cell parameters a= b=99.56, c= 57.19 A
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