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期刊论文

al. Proliferation and pluripotency potential of ectomesenchymal cells derived from first branchial arch

田卫东Yunfeng Lin* Zhengbin Yan* Lei Liu* Ju Qiao† Wei Jing* Ling Wu* Xizhe Chen* Zhiyong Li* Wei Tang* Xiaohui Zheng*and Weidong Tian* ‡ *

Cell Prolif. 2006, 39, 79-92,-0001,():

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tomesenchymal cells in the first branchial arch were enzymatically isolated from the mandibular processes of BALB/c mice and were maintained in an intact state in a medium containing leukaemia inhibitory factor. Here, we first evaluated the proliferative activity of the cells after the third passage, using bromodeoxyuridine labelling and in situ hybridization of telomerase mRNA. Positive staining for expression of HNK-1, S-100 and vimentin confirmed that the population of stem cells originated from the ectomesenchyme, which did not express cytokeratin. Then we investigated the molecular and cellular characteristics of the ectomesenchymal cells during their differentiation towards neurogenic, endothelial, myogenic and odontogenic lineages. Expression of multiple lineagespecific genes and proteins was detected by utilizing a range of molecular and biochemical approaches when the cells were transferred to inductive medium. Histological and immunohistochemical analysis of the induced cells at various intervals indicated obvious phenotypic alteration and presence of specific proteins for the differentiated lineages, for example nestin, factor VIII, α-SMA and dentin sialophosphoprotein (DSPP), respectively. Correlatively, results of reverse transcription–PCR corroborated at Mrna level the expression of the characteristic molecules during differentiation. Therefore, it is suggested that the ectomesenchymal cells derived from the first branchial arch may represent a novel source of multipotential stem cells capable of undergoing expansion and variant differentiation in vitro.

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版权说明:以下全部内容由田卫东上传于   2006年11月09日 19时40分17秒,版权归本人所有。

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