Plasmodium falciparum CTL epitope minigenes containing HLA-A2 and HLA-B7 subtype supermotifs were cooned into a plasmid expression vector, this expression was measured in eight buman HLA class I molecule specific cell lines. Three assays for in vitro antigen prsentation analysis were developed to examine the cross-binding between CTL epitopesand HLA classI molecules, including cell surface peptide-MHC class I binding assay, bindingstabilization assay and MHC class I assembling assay. The results demonstrated that the HLA-B51 restricted CTL epitope of Plasmodium faiciparum could be presented by other HLA class I molecules; however, no other presentation was found for HLA-A2.1 CTL epitope. This work suggests the possibility for improved vaccine-coverage rates by development of a CTL vaccine which contains epitopes capable of cross-binding among different MHC class I alleles.