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期刊论文
6-BA延缓大豆叶片衰老的作用与膜蛋白磷酸化状态的关系
植物生理学报,1998,24 (3): 305~308,-0001,():
The plasma membrane frac-tion isolated from primary leaves of 14 d soybean shoot showed protein kinase (PK) and protein phosphatase (PP) ac- tivities (Fig. 1), and these activities declined dramatically when the leaves underwent senescence-inducing treat- ment (Table 1). 6-BA pretreatment, which showed retarding effect on soy- bean leaf senescence, could sustain the activities of both Pigs and PPs during the senescence-inducing treatment (Table 1). The results of autoradiogra- phy suggested that the main PKs whose activities declined during leaf senes- cence were calcium-dependent protein kinases (Fig. 3). The potent and specif- ic inhibitor of protein phosphatase 1 and 2A, okadaic acid, could fasten leaf senescence. These results mplied that retaining the balance of plasma mem- rane protein phosphorylation/dephos- phorylation was the key action of exoge- nous 6-BA in retarding leaf senescence.
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