Redclaw cray¢sh (Cherax quadricarinatus) females at di¡erent stages of vitellogenesis were sampled to investigate variations in soluble protein, lipid andwater content and in the amino acid and fatty acid composition of the hepatopancreas and ovaries. During vitellogenesis, the changes in the content of soluble proteins and lipids inthe hepatopancreas and ovaries were dependent on both diet and the reserves from the hepatopancreas. However, in comparison to the ovary, the £uctuation of the amino acid composition in the hepatopancreas suggested that the protein resourcesmobilized from this organwas compensating for those gained from feeding. Changes in the fatty acid composition of the hepatopancreas showed imited compensatory function as for the quick accumulation of lipids in the ovaries. The proportional amounts of saturated fatty acids/mono-unsaturated fatty acids/poly-unsaturated fatty acids (PUFA) and the predominant fatty acids in both tissues indicated that the mobilization and utilization of fatty acids in freshwater species are di¡erent from that in marine species. Based on the redclaw’s feeding habits, the commercial pellets used in this study may not be optimal, and a diet with less PUFA may su⁄ce for its vitellogenesis and reduce the feeding costs.