Transmission studies for multiple heterostructures inserted with defects are presented. The results show that the enlargement of the forbidden band and the realization of multiple channeled filtering can be reached simultaneously with these configurations. The frequency, frequency interval, and number of channels can be tuned by adjusting geometric and physical parameters of the defects. Sidebands of the forbidden band can be substantially restricted as compared with that of periodic structures. Experimental fabrication of the designed configurations is carried out with the heterostructures containing TiO2/SiO2 multilayer defects, and the results agree with the calculation very well.