Objective: To investigate the method, indications and initial results of semiconductor diode laser application in tympanoplasty. Method: The diode laser was used in 14 tympanoplasty procedures. The laser ma-nipulation was mainly used in cases of malleus and incus bony fixation with the tympanum, the fibrous scar tissue around the ossicular chain and the malleus head resection. Result: There was no postoperative vertigo and facial paralysis. The average heraing improvement was 24 dB. Seven cases (50%) reached an air-bone gap within 20 dB. Conclusion: The application of diode laser in tympanoplasty could effectively reduce the manipulation trauma of ossicular chain and control the bleeding of operative field, especially in managing the bony adhesion of the os-sicular chain.