您当前所在位置: 首页 > 学者

谢志雄

  • 20浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 182下载

  • 0评论

  • 引用

期刊论文

Escherichia coli is naturally transformable ina novel transformation system

谢志雄

FEMS Microbiol Lett 265 (2006) 249-255,-0001,():

URL:

摘要/描述

A novel transformation system, in which neither a nonphysiological concentration of Ca2+and temperature shifts nor electronic shocks were required, was developed to determine whether Escherichia coli is naturally transformable. In the new protocol, E. coli was cultured normally to the stationary phase and then cultured statically at 37℃ in Luria-Bertani broth. After static culture, transformation occurred in bacteria spread on Luria-Bertani plates. The protein synthesis inhibitor chloramphenicol inhibited this transformation process. The need for protein synthesis in plated bacteria suggests that the transformation of E. coli in this new system is regulated physiologically.

【免责声明】以下全部内容由[谢志雄]上传于[2011年06月27日 15时32分07秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。

我要评论

全部评论 0

本学者其他成果

    同领域成果