您当前所在位置: 首页 > 学者

徐长庆

  • 26浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 33下载

  • 0评论

  • 引用

期刊论文

小檗碱对心肌细胞Ik1、Ik及HERG通道的抑制作用1

徐长庆 LI Bao-Xin YANG Bao-FengZHOU JinXU Chang-Qing LI Yu-Rong

Acta Pharmacol Sin 中国药理学报 2001 Feb, 22 (2): 125-131,-0001,():

URL:

摘要/描述

AIM: To study the effects of berberine on inward rectifier potassium current (Ik1) and outward delayed rectifier potassium current (Ik) of guinea pig ventricular my ocytes, and on human ether-a-go-go related gent (HERG) channel expressed in Xenopus oocytes. METHODS: Whole cell patch-clamp and geneclamp techniques were used to record ionic currents.RESUTS: Berberine prolonged action potential duration (APD) and inhibited Ik1 and Ik in a concentration-dependent manner. Berberine 100μmo1/L increased APD90 from (450±48) ms to (888±90) ms (n=6,P<0.01), and inhibited Ik1 by 64%±7%(n=6,P<0.01). Berberine 50μmo1/L inhibited Ik by 57%±6%,Iktmil by 52%±6%(n=6,P<0.01). Berberine produced a voltage-dependent block on Ik that increased with stronger depolarization, and once all channels activated, there was no further block at positive potentials. Berberine blocked the HERG channels potently with an IC50 value of approximately 75μmo1/L. This block was voltage-dependent, suggesting that it probably bind to either open or inactivated HERG channels. CONCLUSION: Berberine prolonged APD and possessed blocking effect on Ik1,Ik, and HERG channel expressed in Xenopus oocytes. The antiarrhythmic mechanism of berberine is related to its inhibitory effects on Ik1,Ik,and HERG channel.

【免责声明】以下全部内容由[徐长庆]上传于[2007年05月29日 16时01分05秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。

我要评论

全部评论 0

本学者其他成果

    同领域成果