提示
徐顺清
-
84浏览
-
0点赞
-
0收藏
-
0分享
-
295下载
-
0评论
-
引用
期刊论文
Ultrasensitive Detection of Protein Using an Aptamer-Based Exonuclease Protection Assay
Anal. Chem. 2004, 76, 5605-5610，-0001，（）：
Currently, methods for protein detection are not as sensitive and specific as methods for detection of specific nucleic acid sequences. Here, we present an analogous technique for detection of proteins using aptamers as ligands for target binding. We have named this method the aptamer-based exonuclease protection assay. We applied a special oligonucleotide probe containing a thrombin aptamer, which has the capacity to recognize thrombin with high affinity and specificity. The aptamer probe is a 22-base-long single-strand oligonucleotide with the thrombin aptamer sequence at the 3
【免责声明】以下全部内容由[徐顺清]上传于[2005年07月06日 00时36分40秒]，版权归原创者所有。本文仅代表作者本人观点，与本网站无关。本网站对文中陈述、观点判断保持中立，不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考，并请自行承担全部责任。
本学者其他成果
同领域成果
