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期刊论文

Antagonistic action of caffeine against LY294002-induced apoptosis in cerebellar granule neurons1

颜光美WANG Wen-Ya LI Ming-Tao PI Rong-Biao QIU Peng-Xin SU Xing-Wen LIN Sui-Zhen YAN Guang-Mei

Acta Pharmacol Sin 2000 Jun; 21(1):35-40,-0001,():

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摘要/描述

AIM: To study the effect of caffeine on apoptosis induced by inhibition of I-phosphaddylinositol 3-kinase in cerebellur granule neurons. METHODS: Cerebellar granule neurons culture, agar gel eletrophoresis, and tess-activated protein kinase (SAPK)/c-Jun N-tennial, protein kimtse (JNK) assay kit to mesure SAPK/JNK activity. RESULTS: LY294002 evoked apoptosis concentmtion-dependemly in cerebellar granule neurons. But death resulting from LY2940002 was prevented by caffeine in a concentratlon-dependent manner. The survival effect of caffeine was not affected by inbitors of ryanodine-sensitive Ca2+ release, nor was it inhibited by L-type channel blockers and N-methyi-D-aspartate (NMDA) receptor blocker. In addition, RP-cAMP, H89, and KN62 were not able to inhibit the protective effect of caffeine. Phosphorylation of c-Jun was necessary for the induction of apoptosis induced by LY294002 in cerebellar granule neurons. But caffeine lirectly inhibited the activation of JNK and decreased phospho-c-Jun in granule neurons. CONCLUSION: Caffeine inhibited the activation of JNK and decremsed the phosphorylabon of c-Jun to protect granule neurons from LY294002-ieduced apoptosis.

版权说明:以下全部内容由颜光美上传于   2005年03月08日 17时45分17秒,版权归本人所有。

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