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期刊论文
Characterization of Halomonas Varabilis Strain HTG7 Conferring Glyphosate Resistance①
HIGH TECHNOLOGY LETTERS Mar. 2004 Vol. 10 No.1,-0001,():
Bcterial strain HTG7 is isolated from extremely glyphosatepolluted soil. It is identified as Halomonas Varabilis. It can tolerate in 500m mol/L glyphosate concentration. Physiological characteri-zation of strain HTG7 shows that the optimum pH and temperature are 7.0 and 30℃, respectively. It grows well in the NaCl concentrations ranging from 0% to 10%. A plasmid pACYC184 carrying a3.5kb DNA fragment, which confers increased glyphosate tolerance, is cloned. The DNA fragment is able to complement with an E. coli auxotrophic aroA mutant.
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