This study was performed to explore the effect and significance of gastric mucosal histology and ITF mRNA in stress-related rats with lectroacupuncture (EA) on the stomach channel and gallbladder channel. Forty rats were randomly divided into 4 groups: blank control group, model control group, Stomach Meridian of Foot-Yangming(SMFY) group, Gallbladder Meridian of Foot-Shaoyang (GMFY) group. After some procedures, all rats (except normal group) were made model by WRS on day 7. Then the gastric mucosa tissure of each rat was taken off after assessment of gastric mucosal lesion index(GUI), and half of above tissue in each group of was detected the expression of ITF mRNA by Reverse-transcriptase- polymerase chain reaction(RT-PCR) method, meanwhile the gastric mucosal ulcer index（UI） and the histological change and degree of injury of gastric mucosa were observed grossly under light microscope or magnifier. As a result, the UI value in SMFY/ GMFY group decreased obvious or extremly obvious（P﹤ 0.05 or 0.01）; EA had a tendency of improving: The level of ITF mRNA expression of SMFY group was strongly higher than that of model group(P﹤0.01), while that of GMFY group was high than model group but no statistic difference(P﹥0.05); Mucosal hyperaemia and edema but little hemorrhage and gastric ulcer could be observed in group with EA under light microscope, and that of SMFY group was more significant than GMFY. The difference effect of gastric mucosal protection by EA on stomach channel and gallbladder channel, was related to the expression variance of intestinal trefoil factor.