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期刊论文

Microcalorimetric Study on Tyrosine Oxidation Catalyzed by Tyrosinase

姚俊Jun YAO Yi LIU Tian Zhi WANG Jian Ben LIU Peng LIU Zi Niu YU*

Chinese Chemical Letters Vol. 14, No.6, pp 619-622, 2003,-0001,():

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摘要/描述

Through the method of initial heat release rate, the kinetic property of tyrosine oxidation catalyzed by tyrosinase from Pseudomonas maltophilia was investigated using a LKB-2107 batch microcalorimeter. Tyrosine was catalyzed and oxidized into L-dopa, then into melanin catalyzed by tyrosinase. We found that the tyrosinase reaction obeyed the Michaelis-Menten kinetics, and at 298.15K and pH 7.0, the initial exothermic rate (Ω0) are in the range of 0.1567~0.5704mJ·s-1, the maximum exothermic rate (Ωmax) are in 0.4152~0.8143mol·L-1, and mean value of the Michaelis constant (Km) is 2.199 0.105×104 mol L-1.

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