Objective: To observe the effect of Qingkailing (Q KL) on brain damage induced by glutamate, in order to seek for effective drugs for antagonizing neurotoxicity of glutamate. Methods: The number and mor-phological met rology of neurocytes in cerebral cortex and hippocampus were detected by MIAS-300 image anal-yser, elect ron microscope and immunohistochemical methods. Results: Q KL could alleviate the glutamate induced accumulation of water and sodium in brain tissue, relieve the met rological and st uctural damage of cerebral cells in cortex and hippocampus, reduce the percentage of c-fos positive cell in brain. Conclusion: Q KL could protect brain damage induced by glutamate, which might be related to the nhibition of Q KL on the enhancement of c-fos gene expression induced by glutamate.