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期刊论文
Loss of Heterozygosity and its Correlation with Clinical Outcome and Epstein-Barr Virus Infection in Nasopharyngeal Carcinoma
ANTICANCER RESEARCH 21:3021-3030(2001),-0001,():
carcinomas (NPC) were examined by a lleIotype analysis for the ptuposes of detecting potential association between loss of heterozygosity (LOH), clinicopathological parameters and Epstein-Barr virus (EBV) infection. LOH was performed using 257 polymorphic markers on 22 chromosomes. High frequency LOH (≥60%) was observed on 12 chromosome arms including 1p, 2p, 2q, 3p, 3q, 5q, 9p, 9q, 1 lq, 13q, 14q and 17q, with the highest LOH frequency of 91% on 3p. Seventy-three loci presented LOH frequency≥30%; most of these loci clustered on 1p36 p34, 2p25-p24, 3p14-p21, 3p24-p26, 5q11-q14, 5q31-q33, 9p21-p23, 9q33-q34, 11q23-q25, 13q12 q14, 13q31-q33, 14ql3-q11, 14q32 and 19q13. On 1p36-p34, 2p25-p24, 5qI3-q11, 5q31-q33 and 19q13 are reported for the first time. LOH was correlated with specific clinicopathological parameters including tumor T-stage, N-stage, TNM-stage, tumor differentiation and serum antibody titers of IgA against vires capsid antigens (VCA) and early antigen (EA) of EBVin NPC (LOH frequency≥30%). Significantly high LOH frequency was observed on 9p21 (56%) and 19q13 (50%) in NPC with stage T3+T4, while significantly higher LOH frequency was observed on 12p11 (65%) in NPC with stage T1+T2. Significantly higher LOH frequency on 19q13 was also observed in NPC with advanced TNM-stage (Ⅲ+Ⅳ). High fractional allelic loss (FAL) value and high antibody titers of EBV IgA/VCA and/or IgA/EA were significantly correlated with T3+T4-stage, distant lymph node metastasis and advanced TNM-stage of NPC. We also found that NPC patients with high titers of IgA/VCA and IgA/EA showed high LOH frequency on 16q (48%) and 19q13 (48%). These results suggest that LOH on 9p21, 16q and 19q13 may be responsible for the aggressiveness and progression of NPC; there may be an interaction between
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