Coenzyme Q10 (CoQ10), a highly lipophilic compound present in the inner mitochondrial membrane, is essential for production of cellular energy in the form of ATP. CoQ10 is used as a dietary supplement and for treatment of various cardiovascular disorders. Our goal was to compare the CoQ10 levels in Asians following multiple oral doses administered as sustained release or regular tablets. Twenty healthy male volunteers (19-23 years old) were divided into two equal groups. Each subject in Group I received 50mg oral doses of coenzyme Q10 as sustained release tablets once a day for fifteen days, while subject in Group II received 50mg doses of coenzyme Q10 regular tablets. The CoQ10 levels were measured by HPLC-UV (reverse phase ODS column, 10mm, 250×4.6mm; oven temperature 30°C). Mobile phase was constituted by methanol–ethanol 9:1 v/v. Flow ratewas 1.5 ml/min and UV detection was carried out at 275nm. Coenzyme Q9 was used as an internal standard. CoQ10 baseline in the morning was 0.88±0.48mg/l. Following 1 week 50mg/d dosing of CoQ10, plasma CoQ10 concentrations increased to 1.85±1.03mg/l for sustained release tablets and up to 1.37±0.74mg/l for regular tablets. The net increment proportion in AUC for sustained release and regular tablets were 148.26±176.56%, 102.57±130.00%, respectively. Both preparations significantly increased the systemic exposure when compared to endogenous baseline.