您当前所在位置: 首页 > 学者

张学

  • 27浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 62下载

  • 0评论

  • 引用

期刊论文

Interaction of the CDK2-associated protein-1, p12DOC-1=CDK2AP1, with its homolog, p14DOC-1R

张学Waranun Buajeebac Xue Zhangbc Hiroe Ohyamac David Hanc Rudee Surarit* Yong Kimc and David T.W. Wong c

Biochemical and Biophysical Research Communications 315(2004)998-1003,-0001,():

URL:

摘要/描述

Human DOC-1/CDK2AP1 gene encodes a growth suppressor protein of 12kDa (p12DOC-1=CDK2AP1). Recently, p12DOC-1=CDK2AP1 has been shown to associate with cell cycle proteins including CDK2 and DNA olymerase a/primase. It negatively regulates CDK2 activities and suppressesDNAreplication. Therefore, identification of other p12DOC-1=CDK2AP1 interacting proteins might clarify its role in the cell cycle regulation and carcinogenesis. The purpose of this study was to identify additional p12DOC-1=CDK2AP1 interacting proteins using the yeast two-hybrid system. Using human p12DOC-1=CDK2AP1 as a bait in a liver cDNA library screening, cDNA clones identical to human DOC-1R transcript were identified. The interaction between p12DOC-1=CDK2AP1 and p14DOC-1R was verified in vitro and in cells. GST pull-down assay and immunoprecipitation experiments confirmed the interaction between the two proteins. The ritical region for p12DOC-1=CDK2AP1's interaction with p14DOC-1R was defined to amino acids 20-25 by using a series of deletion mutants as baits in the yeast two-hybrid system. Our data indicated that p12DOC-1=CDK2AP1 could associate with its homologous protein, p14DOC-1R.

版权说明:以下全部内容由张学上传于   2005年05月25日 22时27分03秒,版权归本人所有。

我要评论

全部评论 0

本学者其他成果

    同领域成果