According to the sequence of a 352bp fragment-M17 from the suppression subtractive hybridization library of the mutant line Cr3529 of Brass/ca napus, primers were designed and a 1000bp upstream fragrnet was cloned by 5'-RACE, which was joined with the original piece and conposed a 1343bp sequence. According to the 3' and 5' ends sequencess, a pair of primers were designed and the encoding region sequence of this unknown protein was amplified and cloned. Se-quence analyzing showed that it had 80.1% identity with an tmlmown-protein-encoding gene from Arabidopsis thaaliana. Swiss-model was used to predict the structure of the induced protein, and a potential transmembrane helix from site 46 Leu to 66 Phe was identified. GLOBE prediction of globularity showed that this protein possessed a compact globular do-main.