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期刊论文

Direct plantlet regeneration from the tuber of Stachys sieboldii

郑国锠Wei Li Huan-Huan Gao Rong Lu Guang-Qing Guo & Guo-Chang Zheng*

Plant Cell, Tissue and Organ Culture 71: 259-262, 2002.,-0001,():

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摘要/描述

Development of an efficient in vitro plantlet regeneration system from tubers of Stachys sieboldii (Miq.), a traditional Chinese medicinal plant and vegetable, is described. Adventitious shoots with an average of 9.1 (after 4 weeks of incubation) shoots per explant were obtained from 60.4% of tuber explants cultured on Murashige and Skoog (MS) medium containing 1.0mgl−1 thidiazuron (TDZ), and after another 4 weeks of incubation on growth regulator free MS medium, 33.5 shoots per explant were achieved. Subcultures showed results that were similar to those of the initial culture. Excised shoots were rooted both on MS and on half strength MS medium with a frequency of about 95%. Excised shoots were also rooted ex vitro by directly planting them into cups containing sand. Regenerated plants with well developed shoots and roots were successfully transplanted to soil, after which they developed free of abnormalities.

版权说明:以下全部内容由郑国锠上传于   2005年02月25日 18时01分49秒,版权归本人所有。

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