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In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert

郑国锠J. Yang Z. Hu G.Q. Guo & G.C. Zheng

Plant Cell, Tissue and Organ Culture 66: 35-39, 2001.,-0001,():

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摘要/描述

An effective protocol has been developed for plant regeneration from cotyledon explants of Swainsona salsula Taubert (Saline swainsona), a medicinal and agronomic shrub. Adventitious shoots were obtained from 83.2% of cotyledon explants from 3-day seedlings cultured on Murashige and Skoog (MS) medium containing 2.0mgl−1 thidiazuron (TDZ), with an average of 9.3 shoots per explant. Individual elongated shoots were rooted on half strengthMS medium supplemented with 2.0mgl−1 indole-3-butyric acid (IBA), with 59.3% success. Regenerated plants with well developed shoots and roots were successfully transferred to soil, without detectable variants. Histological observation revealed that shoots developed from cotyledon explants via organogenesis, with little callus.

版权说明:以下全部内容由郑国锠上传于   2005年02月25日 18时01分59秒,版权归本人所有。

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