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Simultaneous Detection by Consensus Multiplex PCR of High-and Low-Risk and Other Types of Human Papilloma Virus in Clinical Samples

郑鹏生PENG-SHENG ZHENG*† SHOU-Rou L* TSUYOSHI IWASAKA JIE SONG MAN-HUA Cu AND HAJIME SUGIMORI†

GYNECOLOGIC ONCOLOGY 58. 179-183 (1995),-0001,():

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摘要/描述

A consensus multiplex PCR rCM-PCR)technique was devel-oped to detect high-risk fHPV 16/18), low-risk(HPV 6/11), and over 40 other types of human papillomavirus(HPV), separately but simultaneously. by mixing three pairs of consensus primers in the same PCR mixture, for ene amplification. Simultaneous detection of three groups 0f HPV DNA provides valuable infor-matfon for clinical practice and this procedure is simple and convenient for routine laboratory examinations. We detected HPV DNA sequences in plasmid HPV DNA and DNA extracted from tissues of condyloma acuminata and cervical carcinoma and from exfoliated cells of the lower genital tract of healthy Chinese women living in the People’s Republic of China. We confirmed that this simple, convenient, and cost-beneficial CM-PCR technique is reliable for the detection of HPV DNA se-quences.

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版权说明:以下全部内容由郑鹏生上传于   2009年06月09日 14时02分53秒,版权归本人所有。

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