您当前所在位置: 首页 > 学者

朱培庭

  • 7浏览

  • 0点赞

  • 0收藏

  • 0分享

  • 47下载

  • 0评论

  • 引用

期刊论文

大鼠梗阻性胆管炎细胞免疫功能降低及中药锦红片的影响

朱培庭章学林张静顾宏刚马恩伟

世界华人消化杂志,2007,15(18):2050~2053,-0001,():

URL:

摘要/描述

AIM: To explore the mechanism of decreased cellular immune function and the effect of Jinhong Tablets, a Chinese herbal medicine, during the course of obstructive cholangitis. METHODS: An obstructive cholangitis model was induced in 24 male Sprague-Dawley rats that were randomly divided into model (n=8), Jinhong Tablet-treated (n=8) and simple obstructive cholangitis (n=8)groups. Four days after modeling, all rats were sacrificed and the interleukin (IL)-2, CD3+, CD4+ and CD8+ levels in plasma were measured by enzyme liked immunosorbent assay (ELISA). The endotoxin content was detected by a reagent kit. The index of apoptosis was determ ined by Tdt-mediated flu-orescein-dUTP nick end labeling (TUNEL), and ultrastructure changes of the thymus and apoptosis were observed by electron microscopy. RESULTS: Plasma IL-2, CD3+, and CD4+ levels and the thymus mass index in the untreated group were significantly lower than those in the Jinhong Tablet-treated and simple ligation groups (IL-2: 28.5±3.0 ng/L vs 33. 9土3.6 ng/L, 39.6±2.2ng/L, P<0.05, P<0.01; CD3:54.5%±5.5% vs 70.7%±4.8%, 66.3%±7.1%, both P<0.01; CD4: 34.5% 土8.3% vs 44.2% ±3.3%, 4.5% ±4.2%, both P<0.01; thymus index: 0.89±0.18 vs 1.10±0.13, 1.12±0.24, both P<0.05). There were no significant differences among the three groups with regard to the level of plasma CDR, while plasm a endotoxin and the thymus apoptosis index in the untreated group were significantly higher than in the Jinhong Tablet-treated and simple ligation groups rendotoxin: 0.85±0.14 EU/ml vs 0.53±0.10 EU/mL, 0.49±0.11 EU/ml, both P<0.01; thymus index: 25.7±5.1 vs 15.8土5.5, 9.0±3.1, P<0. 05, P<0. 01). More typical apoptosis cells were seen in thymus tissue than in the untreated group. The results demonstrated that intervention by traditional Chinese medicine improved the immune function, endotoxemia and thymus cell apoptosis, even approaching the levels in the simple obstructive cholangitis. CONCLUSlON: Decreased cellular immune function and abnorm al apoptosis of thymus cells exists in rats with obstructive cholangitis; Jinhong Tablets sign ificantly improve the regulation and maintenance of immune function.

【免责声明】以下全部内容由[朱培庭]上传于[2009年09月06日 14时15分40秒],版权归原创者所有。本文仅代表作者本人观点,与本网站无关。本网站对文中陈述、观点判断保持中立,不对所包含内容的准确性、可靠性或完整性提供任何明示或暗示的保证。请读者仅作参考,并请自行承担全部责任。

我要评论

全部评论 0

本学者其他成果

    同领域成果