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期刊论文
The Karyotypes, C-banding Patterns and AgNORs of Epinephelus malabaricus①
HIGH TECHNOLOGY LETTERS, 2004, Vol. 10. No.2, 1~4,-0001,():
The chromosome specimens of Epinephelus malabaricus (Bloch & Schneider, 1801) are obtained from metaphase of kindney cell by vivi-injection of PHA and culture of colchicines, hypatoic-air drying technique, and then by studying their Giemsa stain, G-bands and AgNORs. The results are as follows: (1) E. malabaricus has a diploid chromosome number of 48 and its karyotype formula is 48t, NF=48, sex chromosome is not found. (2) There is a pair of chromosomes with secondary constriction near the centromere of chromosome t24. (3) 1~4 nucleoli appear in the nucleus of interphase, 55% nuclei has 1 nucleolus and only 2% for 4 nucleoli. (4) AgNORs appear in the chromosome t24 of 50% metaphase, sometimes in the chromosome t5, but not in other chromosomes. (5) The AgNORs polymorphisms are in-dividually specific, 1~4 pairs of the number, and the frequency of 4 AgNORs are lowes. (6) The sec-ondary constrictions and positive C-bands are coincident, close to the centromere of the chromosome, and mass coincident, close to the centromere of the chromosome, and mass constrictive heterochromains appear in that region. (7) All the centromeres of chromosomes are darkly stained C-bands, and the whole arm of chromosome t24 and its centromere are same positive C-bands. (8) The evolutive regulation of the karyotype and the developing mechanism of AgNORs and C-bands are discussed.
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