王金星
一直从事动物生化与分子生物学等方面的研究。
个性化签名
- 姓名:王金星
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学术头衔:
博士生导师
- 职称:-
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学科领域:
动物学
- 研究兴趣:一直从事动物生化与分子生物学等方面的研究。
王金星 博士, 教授, 博士研究生导师,山东大学校聘关键岗教授。学术兼职:中国动物学会理事、兽类学报编委、山东动物学会常务副理事长和山东生化与分子生物学会副理事长。1981年山东师范大学本科毕业,1984年山东大学生物系硕士研究生毕业,毕业后留校工作至今,1998年获南京师范大学博士学位。多次短期赴日本山口大学和九州工业大学、德国奥斯纳布吕克大学和吉森大学、韩国忠北大学等进行短期访问和合作研究。一直从事动物生化与分子生物学等方面的研究。主持和参加国家和省部级研究项目20余项,包括八五和九五攻关、973、863和国家自然科学基金等项目。申请国家发明专利8项,获得授权专利4项。在Molecular Immunology, Developmental Comparative Immunology等国内外刊物发表论文100余篇,曾获教育部科技进步三等奖1项,山东大学优秀科研成果一等奖和二等奖多项。近年来在无脊椎动物先天免疫研究方面取得了较好的进展,特别是在先天免疫的非己识别和效应分子等方面取得了较大进展。率先从中国对虾和家蝇中克隆了对虾素、天蚕素和防御素等多种抗菌肽基因,多数在原核和真核内表达成功。此类分子在医学、农业和水产方面有很大应用前景。
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王金星, Xin-Jun Du a, Jin-Xing Wang a, ∗, Ning Liu a, Xiao-Fan Zhao a, Fu-Hua Li b, Jian-Hai Xiang b
Molecular Immunology 43(2006)1633-1644,-0001,():
-1年11月30日
Peritrophin, one of the components of the peritrophic matrix, was first isolated from the intestine of insects. It is thought to protect insects from invasion of microorganisms and to stimulate digestion of food. Peritrophin-like proteins have also been found in crustaceans, as a component of the egg layer. In this study, one fragment of the peritrophin-like gene was obtained from fleshy prawn (Chinese shrimp) (Fenneropenaeus chinensis) by panning the T7 phage display library constructed with the shrimp hemocyte cDNA. The total sequence of the peritrophin cDNA was cloned by modified SMART cDNA and LD-PCR methods. The full cDNA is 1048 bp and the deduced protein is composed of 274 amino acids, including 21 amino acid signal peptide, and four peritrophin A domains and the latter three forming three chitin-binding domains. Similarity analysis results showed that the peritrophin-like protein from F. chinensis has significant similarities with peritrophin-like and cortical rod proteins from other shrimp. It was inducing expression in hemocytes, heart, stomach, gut, and gills of the infected shrimp, and constitutive expression in the ovaries. No expression signal was detected in the hepatopancreas of either infected or noninfected shrimp. The recombinant peritrophin-like protein has the activity of binding Gram-negative bacteria and strong binding activity to chitin. Therefore, the bacteria and chitin binding activities of the peritrophin-like protein suggest that it may plays a role in immune defense and other physiological resposes.
Peritrophin-like gene, Expression pattern, Fleshy prawn, Fenneropenaeus chinensis, Chitin binding activity, Immune defense
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王金星, JIN-XING WANG, XIAO-FAN ZHAO, HUNG SUN KOH, YI DENG and HONG-YING QI
Hereditas 138: 59-64(2003),-0001,():
-1年11月30日
Different cytogenetic techniques were used to analyze the chromosomes of white-bellied rat, Ni i enter confucianus from Mt. Tai and Jinan, Shandong Province and Ningshan, Shaanxi Province of China. Shandong populations have 2n=46 chromosomes with 4 metacentric, 2 subtelocentric, 16 telocentric pairs of autosomes and the submetacentric X and telocentric Y. The chromosomal arm number (NF) of the two populations was 56. Shaanxi population has 2n=46 chromosomes with 4 metacentric, 1 submetacentric, 1 subtelocentric and 16 telocentric pairs of autosomes and the submetacentric X and telocentric Y. The karyotype of Ningshan population showed NF=58. As the result of the comparison of C-and G-banding patterns, and compare with other species in the genus Ni i enter, we suppose that the chromosomal evolution of Ni i enter involved in pericentric inversion and heterochromatin growth. The submetacentri chromosomes of Shaanxi population would be originated from the growth of heterochromatin of the subtelocentric chromosome of Shandong population.
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【期刊论文】Chromosomal polymorphism of mandarin vole, Microtus mandarinus (Rodentia)
王金星, J. X. WANG, X. F. ZHAO, Y. DENG, H. Y. QI and Z. J. WANG Wang, J. X., Zhao, X. F., Deng, Y., Qi, H. Y. and Wang, Z. J.
Hereditas 138: 47-53(2003),-0001,():
-1年11月30日
The mitotic and meiotic chromosomes of mandarin vole, Microtus mandarinus Milne-Edwards, from Shandong Province of China were analyzed by conventional, G-and C-banding and Silver-staining techniques. We detected chromosomal polymorphism in the vole, exhibiting diploid chromosome numbers 2n=48–50 and variable morphology of the 1st pair, one medium sized telocentric pair and the X chromosomes. Four types of karyotypes were revealed in the population. According to banding analysis, there were pericentric inversion, Robertsonian fusion and translocation in M. mandarinus karyotype evolution. The X displayed two different morphologies, which could be explained by prericentric inversion and a telocentric autosome translocation.
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王金星, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing Wang∗
Molecular Immunology 44(2007)1085-1094,-0001,():
-1年11月30日
Pattern recognition proteins (PRPs), such as lipopolysaccharide and-1,3-glucan binding protein (LGBP), have been identified in many animals and play a crucial role in invertebrate defense systems. In the current study, an LGBP gene was cloned from fleshy prawn (Fenneropenaeus chinensis, Fc-LGBP) utilizing homology cloning and RACE methods. The full cDNA of the Fc-LGBP gene in fleshy prawn was 1253 bp in size with a deduced 366 amino acid protein that includes a glycosyl hydrolase domain. Northern blot and RT-PCR data suggested that Fc-LGBP mRNA was mostly synthesized in haemocytes and that the expression was down-regulated 24 h post-injection of bacteria. In situ hybridization demonstrated that Fc-LGBP mRNA was only detected in haemocyte cytoplasm, with no detection in other tissues. The molecular weight of the purified recombinantly expressed Fc-LGBP was approximately 46 kDa. Immunohistochemistry of haemocytes revealed that Fc-LGBP protein was localized on the membrane of most cells. Data from bacterial binding assays utilizing purified protein suggested that rFc-LGBP had strong binding activity to Gram-negative bacteria.
LGBP, Fleshy prawn, Fenneropenaeus chinensis, Pattern recognition protein, Innate immunity
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王金星, Yun-Dong Sun, Xiao-Fan Zhao, Cui-Jie Kang, Jin-Xing Wang∗
Molecular Immunology 43(2006)1202-1210,-0001,():
-1年11月30日
Thrombospondins (TSPs) are extracellular, multidomain, calcium-binding glycoproteins that modulate cell behavior in homeostasis and during development, wound-healing, immune response and tumor growth of adult tissues in vertebrates. In invertebrates these proteins are a major component of cortical rods in mature oocytes.Afragment of a thrombospondin-like genewas generated by screening a subtractive cDNA library constructed from the hemocytes of Chinese shrimp, Fennerpenaeus chinensis. The full length F. chinensis cDNA of thrombospondin was cloned by 3-and 5-rapid amplification of cDNA ends (3-and 5-RACE). The complete cDNA sequence, named Fc-TSP, is 2886 bp and the open reading frame of the cDNA encodes a 938-residue protein that contains three ChtBD2 domains, an EGF domain, a TSP-3 domain and a common TSP-C (CTD) domain. The protein shares a high sequence identity with the mj-TSPa (46.3%), mj-TSPb (46.9%) and mj-TSPc (51.9%) of Marsupenaeus japonicus. The expression and distribution of Fc-TSP in both challenged and unchallenged shrimps were studied by Northern blot, RT-PCR and in situ hybridization. Northern blot analysis showed that the Fc-TSP transcripts were detected in the hemocytes, heart, intestine, stomach and ovary of both challenged and unchallenged shrimps, but the signal was much stronger in the challenged tissues. A strong hybridization signalwas detected only in challenged hepatopancreas, with no signal in the unchallenged tissue. TheRT-PCR showed that the Fc-TSP was detected in both challenged and unchallenged tissues including the hemocytes, heart, hepatopancreas, stomach, gills, intestine, spermary and ovary. Except for the ovary and spermary, the signal of challenged tissues was relatively stronger than that of unchallenged ones, especially in hepatopancreas. These results suggest that the thrombospondin was upregulated in the hemocytes, heart, intestine and stomach of challenged shrimp, and induced in the hepatopancreas of challenged shrimps. Therefore, Fc-TSP may be involved in the defense responses of the shrimp.
Chinese shrimp, Fennerpenaeus chinensis, Thrombospondin, Expression pattern, Innate immunity
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王金星, Dian-Xiang Li, Xin-Jun Du, Xiao-Fan Zhao, Jin-Xing Wang*
Fish & Shellfish Immunology 21(2006)284-292,-0001,():
-1年11月30日
O-methyltransferase (OMT) is ubiquitously present in diverse organisms and plays an important regulatory role in plant and animal growth, development, reproduction and defence and has also been implicated in human emotion and disease. A putative O-methyltransferase (OMT) gene has been cloned from the haemocytes of bacteria-infected Chinese shrimp (Fenneropenaeus chinensis) by suppression subtractive hybridisation (SSH) coupled with the SMART cDNA method. The isolated 944 bp full-length cDNA contains a single 666 bp open reading frame (ORF) encoding a putative OMT protein of 221 amino acids. The predicted protein has a molecular weight of 24 572.06 Da and a pI of 5.27 as well as ten phosphorylation sites. Northern blot and in situ hybridisation analyses demonstrated that the OMT transcripts were constitutively expressed in tissue of shrimp challenged by bacterial infection and in unchallenged shrimp tissue. Constitutive OMT transcript was found in areas such as haemocytes, heart, hepatopancreas, stomach, gill, intestine and ovary. However, the OMT transcripts were upregulated in hepatopancreas and stomach in challenged shrimp.
O-Methyltransferase, Fenneropenaeus chinensis, Gene cloning, Expression pattern
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王金星, Cui-Jie Kang, Jian-Feng Xue, Ning Liu, Xiao-Fan Zhao, Jin-Xing Wang∗
Molecular Immunology xxx(2006)xxx-xxx,-0001,():
-1年11月30日
Penaeidins are members of a special family of antimicrobial peptides existing in penaeid shrimp and play an important role in the immunological defence of shrimp. Here, we report one penaeidin with a putative isotype newly cloned from fleshy prawn Fenneropenaeus chinensis. The penaeidin open reading frame encodes a 79 amino acid peptide while two exons and an intron were identified within the 1126 bp genomic sequence of Fenchipenaeidin 5. Phylogenetic analysis and sequence comparison with other known penaeidins suggest the new gene belongs to a novel subfamily of penaeidins and the two isoforms were named Fenchi-penaeidin 5-1 and 5-2, respectively. Fenchi-penaeidin 5 mRNA was examined in normal and microbial challenged shrimp and was found to be constitutively expressed in heamocytes, heart, gill, intestine and ovary. Bacterial challenge resulted in mRNA up-regulation, inducing expression in hepatopancreas and stomach. Fenchi-penaeidin 5-1 was also expressed in Pichia pastoris, and recombinant Fenchi-penaeidin 5-1 exhibited activities against Gram-positive and -negative bacteria and fungi.
Fleshy prawn, Fenneropenaeus chinensis, Antimicrobial peptide, Penaeidins, Gene expression, Pichia pastoris
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王金星, Lei Li a, Jin-Xing Wang a, *, Xiao-Fan Zhao a, Cui-Jie Kang a, Ning Liu a, Jian-Hai Xiang b, Fu-Hua Li b, Shinji Sueda c, Hiroki Kondo c
Protein Expression and PuriWcation 39(2005)144-151,-0001,():
-1年11月30日
Penaeidins, members of a new family of antimicrobial peptides constitutively produced and stored in the haemocytes of penaeid shrimp, display antimicrobial activity against bacteria, and fungi. Here, a DNA sequence encoding the mature Ch-penaeidin peptide was cloned into the pPIC9K vector and transformed into Pichia pastoris. The transformed cells were screened for multi-copy plasmids using increasing concentrations of G418. Positive colonies carrying chromosomal integrations of the Chp gene were identiWed by phenotype and PCR. When transformed cells were induced with methanol, SDS-PAGE and Western blotting revealed the production of a
Chinese shrimp (, Fenneropenaeus chinensis), , Antimicrobial peptide, Pichia pastoris, Recombinant expression, Penaeidin
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王金星, Cui-Jie Kang a, Jin-Xing Wang a, ), Xiao-fan Zhao a, Xiao-Mei Yang a, Hong-Lian Shao a, Jian-Hai Xiang b
Fish & Shellfish Immunology 16(2004)513-525,-0001,():
-1年11月30日
A new member of antimicrobial peptide genes of the penaeidin family, Ch-penaeidin, has been cloned from the haemocytes of Chinese shrimp, Fenneropenaeus chinensis, by reverse transcription PCR (RT-PCR), 3#-rapid amplification of cDNA end (3#-RACE) and smart cDNA methods. The Ch-penaeidin cDNA was 655 bp and the open reading frame of the cDNA encoded a 71 amino acid peptide. Ch-penaeidin contained a putative NH2-terminal signal sequence (1e19) followed by a mature peptide (20e71). The sequence identity with other penaeidins from Litopenaeus vannamei and Litopenaeus setiferus is between 48% and 71%. The signal sequence of Ch-penaeidin is almost completely identical to that of other penaeidins, while differing relatively in the N-terminal domain of the mature peptide. Ch-penaeidin was designated as a novel member of class penaeidin 3 according to phylogenetic analysis. The mature peptide, with a predicted molecular weight of 5589.32 Da, and a pI of 9.77, has eight positively charged amino acids and no negatively charged amino acids. The expression and distribution of Ch-penaeidin in unchallenged shrimps were studied by RT-PCR, Northern blot and in situ hybridisation. The results showed that the Ch-penaeidin transcripts were detected in haemocytes (granular haemocytes), heart, gill, intestine, and subcuticular epithelia of the shrimp, and that Ch-penaeidin was constitutively expressed mainly in haemocytes.
Fenneropenaeus chinensis, Penaeidin, Antimicrobial peptides, Gene expression, Innate immunity
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王金星, Yongli Liang, Jin-Xing Wang*, Xiao-fan Zhao, Xin-Jun Du, Jian-Feng Xue
Developmental and Comparative Immunology 30(2006)249-257,-0001,():
-1年11月30日
A cDNA encoding housefly (Musca domestica) cecropin transcript was isolated from total RNA using RT-PCR, 30RACE, and lgt11 cDNA library screening, and was expressed in Escherichia coli. This is the first report of a cecropin nucleotide and amino acid sequence in the housefly. The open reading frame of Md-Cec (189 bp) encodes a precursor of 63 aa, which is comprised of a 23 aa signal peptide and a 40 aa mature peptide. In terms of amino acid sequence, Md-Cec shares a high degree of identity (74–82%) with those of some Diptera insects. Northern blot, RT-PCR and in situ hybridization analyses revealed that the prececropin was temporally expressed 5 h after bacteria-challenge in larvae, and was induced in the fat body, epithelia of the body wall, and the epidermis of the midgut. The DNA fragment encoding mature Md-Cec was then subcloned into the pGEX-4T-1 expression vector and was highly expressed in E. coli BL21 with IPTG induction. The expressed proteins, fused to glutathion S-transferase, were purified by glutathion-Sepharose 4B affinity chromatography and cleaved with thrombin, followed by gel filtration chromatography. Recombinant Md-Cec exhibited antimicrobial activity against E. coli.
Cecropin, Housefly (, Musca domestica), , Antimicrobial peptides, RT-PCR, in situ hybridization, Insect innate immunity
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