黄鹰
从事基因工程菌在发酵过程中的稳定性的研究
个性化签名
- 姓名:黄鹰
- 目前身份:
- 担任导师情况:
- 学位:
-
学术头衔:
博士生导师
- 职称:-
-
学科领域:
物理海洋学
- 研究兴趣:从事基因工程菌在发酵过程中的稳定性的研究
黄鹰,男, 1965年8月出生,1986年7月毕业于华东理工大学生物化学工程系生物化学工程专业,获工程学学士学位。毕业后在中科院上海生物工程实验基地工作,主要从事基因工程菌在发酵过程中的稳定性的研究。1997年10月毕业于美国德州大学卫生研究中心圣安东尼奥分校生物化学系生物化学专业,获博士学位。读博期间,阐明噬菌体T7 RNA聚合酶区别底物(rNTP)和非底物(dNTP)的机理,发表3篇论文。博士毕业后在美国国立卫生研究院(NIH)工作期间,研究tRNA生物合成和加工的机理,发表10篇论文。主要贡献包括:纯化和鉴定RNA聚合酶III及其转录因子TFIIIB和TFIIIC,发现tRNA前体3’末端寡聚屎苷酸的长度是受RNA聚合酶III的外切酶活力控制,和人自主抗体La蛋白除具有保护tRNA前体免受核酸外切酶降解,还具有促进有缺陷的tRNA前体成熟的功能。从2006年11月起任南京师范大学生命科学学院微生物系教授。主要以粟酒裂殖酵母为模型系统,研究tRNA生物合成和加工的机理、La蛋白的结构和功能之间的关系、酵母基因沉默的机制,并使用基因组学方法研究粟酒裂殖酵母中所有必需基因的功能。
-
主页访问
2235
-
关注数
0
-
成果阅读
998
-
成果数
18
黄鹰
中国科学C 辑:生命科学,2009,39(1):98~108,-0001,():
-1年11月30日
tRNA是蛋白质翻译机器中的必需成分,它对细胞的生长和增殖及器官的发育起着决定性作用。tRNA生物合成包括tRNA基因的转录、转录后的加工和修饰。对tRNA生物合成的研究还包括tRNA在细胞中的运输、tRNA生物合成的质量监控及其与其他重要细胞途径(如mRNA生物合成、DNA损伤应答和细胞周期)之间的相互作用,以及tRNA生物合成在生长发育和疾病中的作用。本文主要介绍了近年来真核生物细胞质tRNA生物合成研究的一些重要进展。
Trna, tRNA生物合成, tRNA基因转录, tRNA加工, tRNA修饰
-
161浏览
-
0点赞
-
0收藏
-
0分享
-
208下载
-
0评论
-
引用
黄鹰, 于莹莹, 杨景
中国生物化学与分子生物学报,2008,24(8):692~697,-0001,():
-1年11月30日
芽殖酵母(Saccharomycescerevisiae)和裂殖酵母(Schizosaccharomycespombe)是用来研究异染色质形成、细胞周期、DNA复制等重要细胞功能的理想单细胞真核生物。本文主要介绍这2种酵母中异染色质形成的机制。异染色质是一种抑制基因转录和DNA重组的特殊染色质结构。尽管在芽殖酵母和裂殖酵母中异染色质形成都需要组蛋白修饰,但异染色质建立的机制不同。在芽殖酵母中参与异染色质形成的主要蛋白是Sir124蛋白(其中Sir2为组蛋白H3去乙酰化酶),而组蛋白H3赖氨酸9甲基化酶Clr4和异染色质蛋白Swi6在裂殖酵母异染色质形成中起关键的作用。在这两个酵母中,参与异染色质形成的组蛋白修饰蛋白由DNA结合蛋白招募到异染色质。此外,裂殖酵母也利用RNA干扰系统招募组蛋白修饰蛋白。
酵母, 表观遗传, 异染色质, RNA干扰, 组蛋白修饰
-
38浏览
-
0点赞
-
0收藏
-
0分享
-
168下载
-
0评论
-
引用
黄鹰, 杨景, 于莹莹, 黄鹰*
生命科学,2008,20(2):190~195,-0001,():
-1年11月30日
tRNA在蛋白质合成过程中起着极其重要的作用。在所有的生物体内,tRNA首先以前体形式转录,然后必需经过一系列的加工后才能成为有功能的tRNA分子。tRNaseZ、RNaseP和tRNA剪接内切酶是参与tRNA前体加工的三种主要的核酸内切酶,分别参与tRNA前体3'末端、tRNA前体5'末端和内含子剪接的加工。这三种酶具有不同的结构特征,并且利用完全不同的催化机制水解磷酸二酯键。tRNaseZ和RNaseP都是金属酶,活性中心分别需要Zn2+和Mg2+的参与;而tRNA剪接内切酶活性中心不需要金属离子,是一个由不同催化亚基上的关键氨基酸残基构成的组合式活性中心。
tRNA, tRNase Z, RNase P, tRNA 剪接内切酶
-
74浏览
-
0点赞
-
0收藏
-
0分享
-
84下载
-
0评论
-
引用
黄鹰, Ying Huang, Robert V. Intine, Amy Mozlin, Samuel Hasson, and Richard J. Maraia*
MOLECULAR AND CELLULAR BIOLOGY, Jan. 2005, p. 621-636,-0001,():
-1年11月30日
Termination by RNA polymerase III (Pol III) produces RNAs whose 3 oligo(U) termini are bound by La protein, a chaperone that protects RNAs from 3 exonucleases and promotes their maturation. Multiple reports indicate that yeasts use La-dependent and -independent pathways for tRNA maturation, with defective pre-tRNAs being most sensitive to decay and most dependent on La for maturation and function. The Rpc11p subunit of Pol III shows homology with the zinc ribbon of TFIIS and is known to mediate RNA 3 cleavage and to be important for termination. We used a La-dependent opal suppressor, tRNASerUGAM, which suppresses ade6-704 and the accumulation of red pigment, to screen Schizosaccaromyces pombe for rpc11 mutants that increase tRNA-mediated suppression. Analyses of two zinc ribbon mutants indicate that they are deficient in Pol III RNA 3 cleavage activity and produce pre-tRNASerUGAM transcripts with elongated 3 -oligo(U) tracts that are better substrates for La. A substantial fraction of pre-tRNASerUGAM contains too few 3 Us for efficient La binding and appears to decay in wild-type cells but has elongated oligo(U) tracts and matures along the La-dependent pathway in the mutants. The data indicate that Rpc11p limits RNA 3 -U length and that this significantly restricts pre-tRNAs to a La-independent pathway of maturation in fission yeast.
-
71浏览
-
0点赞
-
0收藏
-
0分享
-
70下载
-
0评论
-
引用
黄鹰, Ying Huang, Edward McGillicuddy, Michael Weindel, Steven Dong and Richard J. Maraia*
Nucleic Acids Research, 2003, Vol. 31, No.8 2108-2116,-0001,():
-1年11月30日
The RNA polymerase (pol) III-transcribed (e.g. tRNA and 5S rRNA) genes of traditionally studied organisms rely on gene-internal promoters that precisely position the initiation factor, TFIIIB, on the upstream promoter-less DNA. This is accomplished by the ability of the TFIIIB subunit, TFIIB-related factor (Brf1), to make stable protein±protein interactions with TATA-binding protein (TBP) and place it on the promoter-less upstream DNA. Unlike traditional model organisms, Schizosaccharomyces pombe tRNA and 5S rRNA genes contain upstream TATA promoters that are required to program functional pol III initiation complexes. In this study we demonstrate that S.pombe (Sp)Brf does not form stable interactions with TBP in the absence of DNA using approaches that do reveal stable association of TBP and S.cerevisiae (Sc)Brf1. Gel mobility analyses demonstrate that a TBP±TATA DNA complex can recruit SpBrf to a Pol III promoter. Consistent with this, overproduction of SpBrf in S.pombe increases the expression of a TATA- ependent, but not a TATA-less, suppressor tRNA gene. Since previous whole genome analysis also revealed TATA elements upstream of tRNA genes in Arabidopsis, this pathway may be more widespread than appreciated previously.
-
110浏览
-
0点赞
-
0收藏
-
0分享
-
66下载
-
0评论
-
引用
黄鹰, Ying Huang, Mitsuhiro Hamada, and Richard J. Maraia‡
Vol. 275, No. 40, Issue of October 6, pp. 31480-31487, 2000,-0001,():
-1年11月30日
Eukaryotic tRNA genes are controlled by proximal and downstream elements that direct transcription by RNA polymerase (pol) III. Transcription factors (TFs) that reside near the initiation site are related in Saccharomyces cerevisiae and humans, while those that reside at or downstream of the B box share no recognizable sequence relatedness. Human TFIIICb is a transcriptional regulator that exhibits no homology to S. cerevisiae sequences on its own. We cloned an essential Schizosaccharomyces pombe gene that encodes a protein, Sfc6p, with homology to the S. cerevisiae TFIIIC subunit, TFC6p, that extends to human TFIIICb. We also isolated and cloned S. pombe homologs of three other TFIIIC subunits, Sfc3p, Sfc4p, and Sfc1p, the latter two of which are conserved from S. cerevisiae to humans, while the former shares homology with the S. cerevisiae B boxbinding homolog only. Sfc6p is a component of a sequence-specific DNA-binding complex that also contains the B box-binding homolog, Sfc3p. Immunoprecipitation of Sfc3p further revealed that Sfc1p, Sfc3p, Sfc4p, and Sfc6p are associated in vivo and that the isolated Sfc3p complex is active for pol III-mediated transcription of a S. pombe tRNA gene in vitro. These results establish a link between the downstream pol III TFs in yeast and humans.
-
35浏览
-
0点赞
-
0收藏
-
0分享
-
62下载
-
0评论
-
引用
【期刊论文】Control of Transfer RNA Maturation by Phosphorylation of the Human La Antigen on Serine 366
黄鹰, Robert V. A. Intine, Amy L. Sakulich, Shashi B. Koduru, Ying Huang, Erik Pierstorff, John L. Goodier, Lon Phan, and Richard J. Maraia*
Molecular Cell, Vol. 6, 339-348, August, 2000, Copyright,-0001,():
-1年11月30日
-
32浏览
-
0点赞
-
0收藏
-
0分享
-
55下载
-
0评论
-
引用
【期刊论文】Mechanism of Ribose 2¢-Group Discrimination by an RNA Polymerase†
黄鹰, Ying Huang, ‡ Fritz Eckstein, § Robert Padilla, ‡ and Rui Sousa*, ‡
Biochemistry 1997, 36, 8231-8242,-0001,():
-1年11月30日
The mechanism by which T7 RNA polymerase (RNAP) discriminates between rNTP and dNTP substrates has been characterized. During transcript elongation T7 RNAP uses rNTPs 70-80-fold more efficiently than dNTPs. Discrimination of the hydrogen-bonding character of the ribose 2¢-substituent contributes a largely Km-mediated factor of 20 to this preference for rNTPs. Discrimination of 2¢- substituent H-bonding character appears to be made through a hydrogen bond to the hydroxyl group of tyrosine 639. This hydrogen bond makes little net contribution to either rNTP ground or transition state binding energy apparently because it is balanced by the energy of desolvation of the tyrosine hydroxyl. This mechanism may reflect a strategy to facilitate translocation by minimizing contributions from polymerase-NMP moiety interactions to NTP binding energy so as to minimize the affinity of the NTP binding site for the 3¢-NMP of the product nucleic acid.
-
76浏览
-
0点赞
-
0收藏
-
0分享
-
53下载
-
0评论
-
引用
黄鹰, Ying HUANG, Preston N. GARRISON and Larry D. BARNES*
Biochem. J. (1995) 312, 925-932 (Printed in Great Britain),-0001,():
-1年11月30日
-
36浏览
-
0点赞
-
0收藏
-
0分享
-
34下载
-
0评论
-
引用
-
53浏览
-
0点赞
-
0收藏
-
0分享
-
122下载
-
0评论
-
引用