宗敏华
生物催化新型绿色可再生液体替代能源生物柴油的生产、手性药物及其中间体的生物合成或动力学拆分、生物催化油脂改性、非天然化合物的生物合成与转化、酶法改造天然生物大分子的结构、生物催化药物修饰及非水相生物催化理论方面
个性化签名
- 姓名:宗敏华
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学术头衔:
博士生导师
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学科领域:
生物化学工程
- 研究兴趣:生物催化新型绿色可再生液体替代能源生物柴油的生产、手性药物及其中间体的生物合成或动力学拆分、生物催化油脂改性、非天然化合物的生物合成与转化、酶法改造天然生物大分子的结构、生物催化药物修饰及非水相生物催化理论方面
宗敏华,女, 1960年生,博士,教授,博士生导师。华南理工大学生物科学与工程学科学科带头人。兼任教育部化学与化工学科制药工程专业教学指导委员会委员。主要从事生物催化、生物合成与转化等方面的研究工作,先后主持国家自然科学基金、教育部留学回国人员基金、高校博士点基金、广东省自然科学基金,广东省科技计划项目、教育部重点项目10项。在生物催化新型绿色可再生液体替代能源生物柴油的生产、手性药物及其中间体的生物合成或动力学拆分、生物催化油脂改性、非天然化合物的生物合成与转化、酶法改造天然生物大分子的结构、生物催化药物修饰及非水相生物催化理论方面进行了较系统的研究。在国内外著名刊物上发表论文100余篇,其中被SCI收录30余篇。申请中国发明专利4项。主编由科学出版社出版的《现代化工辞典》(生物化工部分)。为Biotechnology Progress, Journal of Molecular Catalysis B: Enzymatic, Process Biochemistry, Chinese Journal of Chemistry等国内外著名刊物的审稿人。
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宗敏华, Di-Heng Luo a, Min-Hua Zong a, *, Jian-He Xu b
Journal of Molecular Catalysis B: Enzymatic 24-25(2003)83-88,-0001,():
-1年11月30日
A new isolate, Rhodotorula sp. AS2.2241, capable of reducing acetophenone and α-bromoacetophenone with high stereoselectivity, was further studied to exploit its potential in the asymmetric reduction of silicon-containing ketones to silyl alcohols. After encapsulation, the cells were used in an aqueous or an aqueous/organic solvent biphasic system for the asymmetric reduction of cetyltrimethylsilane (ATMS) to prepare (−)-1-trimethylsilylethanol [(−)-TMSE]. It has been found that higher product yield and product enantiomeric excess could be achieved with immobilized cells in an aqueous/organic solvent biphasic system. For optimization of the reaction, various influential variables, such as the volume ratio of the aqueous phase to the organic phase, the hydrophobicity of the organic solvent, reaction temperature, buffer pH of the aqueous phase and the shaking speed, were examined with respect to the initial reaction rate, the product yield and the enantiomeric excess (e.e.) of TMSE formed. All the factors mentioned above had influences on the reaction to some extent. Isooctane was found to be the most suitable organic phase for the reaction. The optimum volume ratio of the aqueous phase to the organic phase, reaction temperature, buffer pH and shaking speed were 1/1, 40℃, 6.5 and 150 rpm, respectively under which the product yield and the product enantiomeric excess were as high as 99 and 90%, which are much higher than those previously obtained by enantioselective reduction of acylsilanes.
Rhodotorula sp., , Asymmetric reduction, Acetyltrimethylsilane, (, −), -1-Trimethylsilylethanol, Aqueous/, organic solvent biphase
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【期刊论文】(R)-Oxynitrilase-catalyzed synthesis of (R)-2-trimethylsilyl-2-hydroxyl-ethylcyanide
宗敏华, Ning Li, Min-Hua Zong*, Hua-Song Peng, Hua-Chang Wu, Chen Liu
Journal of Molecular Catalysis B: Enzymatic 22(2003)7-12,-0001,():
-1年11月30日
The enantioselective synthesis of (R)-2-trimethylsilyl-2-hydroxyl-ethylcyanide with (R)-oxynitrilase from defatted almond meal in a biphasic system was successfully performed. The influences of some factors on the reaction were investigated systematically. Diisopropyl ether was found to be the best for this reaction among all the organic solvents explored. The optimal aqueous phase content, concentrations of crude enzyme, acetyltrimethylsilane, acetone cyanohydrin, shake speed, buffer pH, reaction temperature were 13% (v/v), 5% (w/v), 0.02M, 0.04M, 150rpm, 5.0, 40℃, respectively, under which the initial reaction rate, substrate conversion and product e.e. were 5.87mmol/lh, 99.5 and 99.0%.
(, R), -2-Trimethylsilyl-2-hydroxyl-ethylcyanide, (, R), -Oxynitrilase, Asymmetric synthesis, Organosilicon, Cyanohydrin
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【期刊论文】ENZYMATIC CONVERSION OF WASTE OIL TO BIODIESEL IN A SOLVENT-FREE SYSTEM
宗敏华, Hong Wu, Min-hua Zong*, Qian Luo, and Hua-chang Wu
Prepr. Pap.-Am. Chem. Soc., Div. Fuel Chem. 2003, 48 (2), 533,-0001,():
-1年11月30日
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宗敏华, Wen-Yong Lou, Min-Hua Zong*, Yuan-Yuan Zhang, Hong Wu
Enzyme and Microbial Technology 35(2004)190-196,-0001,():
-1年11月30日
Synthesis of optically active organosilyl alcohol via asymmetric reduction of prochiral acyl silane with immobilized Saccharomyces cerevisiae type II (a mutant from S. cerevisiae by UV irradiation treatment, named UVSC II) cells compared to free UVSC Ⅱcells was studied. It has been found that higher yield and enantiomeric excess (e.e.) of the product could be achieved with immobilized UVSC Ⅱ cells. To further enhance the product yield and the product e.e., an aqueous/organic biphasic system was used as the reaction medium. The influence of various factors, such as shake speed, organic solvent, volume ratio of water phase to organic phase, buffer pH of water phase, reaction temperature and substrate concentration, was systematically studied and the reaction conditions were optimized with respect to the initial reaction rate, the product yield and the product e.e. All the above-mentioned factors had effect on the reaction to some extent. n-Hexane was found to be the best organic solvent. The optimum shake speed, volume ratio of water phase to organic phase, buffer pH, reaction temperature and substrate concentration were 150rpm, 1/2, 8.0, 30℃ and 42mM, espectively, under which the product yield and the product e.e. were as high as 97.4 and 95.4%.
Saccharomyces cerevisiae, Asymmetric reduction, Acetyltrimethylsilane, (, −), -1-Trimethylsilylethanol
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【期刊论文】Lipase-catalyzed Kinetic Resolution of Racemic 1-Trimethylsilylethanol in Organic Solvent*
宗敏华, WU Hong, ZONG Minhua**, WANG Jufang, LUO Diheng and LOU Wenyong
Chinese J. Chem. Eng., 12 (3) 421-424 (2004),-0001,():
-1年11月30日
The enantioselective esterification of racemic 1-trimethylsilylethanol with acids actalyxed by lipase in organic solvent was successfully performed. The influence of some factors on the reaction was investigate. Among the four lipases explores, Candida rugosa lipase (CRL) showed the highest activity and enantioselectivity. Octanoc acid was the best acyl donor among the eleven acids studied and n-hexane was the most suitable medium for the reaction. The optimum shaking rate and temperature were found to be 150r·min-1 and 20℃ to 30℃, respectively. The enantiomeric excess of the remaining (S)-(-)-1-trimethylsilylethanol was 93% when substrate conversion was 53% upon incubation of the reaction mixture at 30℃, 150r·min-1 for 12h.
Candida rugosa lipase,, enantioselective esterification,, kinetic resolution,, racemic 1-trimethylsilylethanol
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宗敏华, Ning Li, Min-Hua Zong*, Chen Liu, Hua-Song Peng & Hua-Chang Wu
Biotechnology Letters 25: 219-222, 2003.,-0001,():
-1年11月30日
Optically active 2-trimethylsilyl-2-hydroxyl-ethylcyanidewas prepared by enzymatic enantioselective transcyanation of acetyltrimethylsilane with acetone cyanohydrin in a biphasic system at 35℃ and pH 5. (R)-Oxynitrilase from apple seed meal was the best among all the enzymes explored and diisopropyl ether was the most suitable organic phase. Acetyltrimethylsilane was a better substrate of the enzyme than its carbon analogue. The substrate conversion and product enantiomeric excess of 2-trimethylsilyl-2-hydroxyl-ethylcyanide were >99% and >99%, respectively.
asymmetric synthesis,, cyanohydrin,, organosilicon,, (, R), -oxynitrilase,, 2-trimethylsilyl-2-hydroxylethylcyanide
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宗敏华, Wei Du, *, Minhua Zong, Yong Guo & Dehua Liu
Biotechnology Letters 25: 461-464, 2003.,-0001,():
-1年11月30日
Lipase-catalyzed enantioselective ammonolysisof phenylglycine methyl ester was processed by in situ racemization with ammonium carbamate as the acyl acceptor. Using 1mMbenzaldehyde or 0.6mM chloropyridozal as the racemizing catalyst, 80% substrate conversion with an enantiomeric excess of the product of 95% were achieved at 20℃ after 7h reactin.
ammonolysis,, enantioselective,, in situ racemization,, lipase,, phenylglycine methyl ester
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宗敏华, Wenyong Lou*†, Minhua Zong* and Hong Wu*
Biotechnol. Appl. Biochem. (2004) 41. 1-6 (printed in Great Britain),-0001,():
-1年11月30日
The aqueous solution of ionic liquid BMIM. BF, (I-butyl-3-methylimidazolum terafluoroborate) was used as the reaction medium for the asymmetric chydrolysis of D, L-p-hydroxyphenylglycine methyl ester to enantiopure L-HPG (L-p-hydroxyphenylglycine) for the first time. Papain was screened out from five protesases and two lipases tested due to its high activity and enantioselectivity for this reaction. comparativestudy demonstrated that papain is more active, enantioselectiveand stable in the sodium phosphate buffer solution of BMIM. BF, than in aqueous buffer and in the bufer solutions of several typical organic solvents examined. L-HPG with the e.e.(enantiomeric examined. L-HPG with the e.e (enantiomeric excess) of 95.6% and the yield of 47.4% (on a mole basis) could be obtained when the reaction was performed in the buffer solution of 12.5% (v/v) BMIM ·BF, (hpH 7.0). The aqueous-ionic liquid co-solvent increased papain's half-life by 3.1-, 3.8-, 4.7-and I 6.3-fold when compared with aqueous buffer, aqueous 2-propanol, aqueous acetonitrile and aqueous I, 4-dioxan co-solvent respectively at 40℃. When the hydrolysis was conducted under reduced pressure, the yield, the e.e. of the L-HPG and the e.e. of remaining D-hydroxyphenylglycine methyl ester achieved were as high as 49.8, 98.1 and>99.0% respectively.
aqueous-ionic liquid co-solvent,, asymmetric hydrolysis,, I-butyl-3-methylimidazolum ter5afluoroborate,, L-p-hydroxyhenylglycine,, papain.,
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宗敏华, WEN-YONG LOU, MIN-HUA ZONG* and HONG WU
Biocatalysis and Biotransformation, 2004 VOL. 22 (3). pp. 171-176,-0001,():
-1年11月30日
Papain-mediated asymmetric hydrolysis of D, L-p-hydroxyphenylglycine methyl ester (D,L-HPGME) was examined in the ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM /BF4) and different solvents. The activity of the enzyme varied widely with change in BMIM /BF4 concentration, with 12.5% (v/v) being the optimum BMIM /BF4 concentration for the reaction. Papain displayed much higher hydrolytic activity and enantioselectivity in phosphate buffer solution of 12.5% (v/v) BMIM /BF4 (pH 7.0) than in other media examined. Comparative studies on the kinetics and activation energy (Ea) of this reaction performed in different media showed a higher Vmax, a lower Km and a lower Ea for the reaction taking place in phosphate buffer solution of 12.5% (v/v) BMIM /BF4 than in other media tested. The stability of papain at 458C was considerably enhanced in BMIM /BF4 as compared with aqueous buffer, 2-propanol and acetonitrile. A half-life time of 169 h was observed with BMIM /BF4 in the presence of substrate, which was 9.2/16.8-fold higher than those with the other solvents. These results suggested that BMIM /BF4 is an excellent reaction medium for this reaction.
Ionic liquid, D,, L-p-Hydroxyphenylglycine methyl ester, Asymmetric hydrolysis, 1-Butyl-3-methylimidazolium tetrafluoroborate, Papain
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宗敏华, Ruo Xu. Min-Hua Zong. Yu-Ying Liu. Jun He. Yuan-Yuan Zhang. Wen-Yong Lou
Appl Microbiol Biotechnol (2004) 66: 27-33,-0001,():
-1年11月30日
(S)-Hydroxynitrile lyase from Manihot esculenta (MeHNL) was shown for the first time to be able to catalyze the enantioselective transcyanation of acetyltrimethylsilane (ATMS) with acetone cyanohydrin to form (S)-2-trimethylsilyl-2-hydroxyl-propionitrile in an aqueous/organic biphasic system. To better understand the reaction, various influential variables were examined. The most suitable organic phase, optimal buffer pH, aqueous phase content, shaking rate, temperature, concentration of ATMS, acetone cyanohydrin and crude enzyme were diisopropyl ether (DIPE), 5.4, 13% (v/v), 190rpm, 40℃, 10mM, 20mM, and 35U/ml, respectively, under which the initial reaction rate, substrate conversion and product enantiomeric excess (e.e.) were 19.5 mM/h, 99.0% and 93.5%, respectively. A comparative study demonstrated that silicon atoms in the substrate had a great effect on the reaction, and that ATMS was a much better substrate for MeHNL than its carbon analogue 3,3-dimethyl-2-butanone (DMBO) with respect to the initial reaction rate, substrate conversion and product e.e. MeHNL has greater affinity towards ATMS than its carbon analogue as indicated by the much lower Km. The activation energy of MeHNL-catalyzed transcyanation of ATMS was also markedly lower than that of DMBO. The silicon effect on the reaction was rationalized on the basis of the special characteristics of silicon atoms and the catalytic mechanism of MeHNL.
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