万建民
水稻的基因定位和克隆研究
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- 姓名:万建民
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博士生导师
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学科领域:
农艺学
- 研究兴趣:水稻的基因定位和克隆研究
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【期刊论文】利用回交重组自交群体检测水稻抗褐飞虱数量性状基因座
万建民, 苏昌潮, 程遐年, 翟虎渠, 万建民①
遗传学报,29 (4): 332~338, 2002,-0001,():
-1年11月30日
利用由98个家系组成的Nipponbare/Kasalath∥Nipponbare回交重组自交系(Backcross Inbred lines,BILs)作图群体(BC1F9),进行水稻抗褐飞虱数量性状基因座(Quantative Trait Locus, QTL)的检测和遗传效应分析。采用苗期集团鉴定方法,并以死苗率作为抗褐飞虱表型值,分析亲本和98个BILs的抗褐飞虱表现。共检测到3个苗期抗褐飞虱QTL,分别位于2、10和12染色体上。各QTL的LOD值为2101~2133,贡献率为1014%~1616%,可解释群体总表型变异的3910%。这3个数量性状基因座对褐飞虱的抗性均来自抗虫亲本Kasalath。与这些数量性状基因座连锁的分子标记可望应用于聚合多个抗性基因,培育对褐飞虱具有水平抗性水稻新品种的育种实践中。
水稻, 抗褐飞虱, 回交重组自交(, BIL), 群体, 数量性状基因座(, QTL), 分析
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万建民, Zefu Li), Jianmin Wan*), Jiafa Xia) and Masahiro Yano)
Breeding Science 53: 209~215 (2003),-0001,():
-1年11月30日
Backcross inbred lines (BILs) derived from a cross of Nipponbare (japonica) / Kasalath (indica) // Nipponbare were used to identify quantitative trait loci (QTLs) controlling physico-chemical properties of rice grains such as amylose content (AC), alkali spreading score (ASS) and gel consistency (GC) by composite interval mapping over a period of two years. A total of 4 QTLs for AC were detected; qAC-5 and qAC-6 showed significant effects (hereafter referred to as “significant”) in both years, and qAC-6 explained more than 80 % of the phenotypic variance and was located in the wx region on the short arm of chromosome 6. The other 2 QTLs for AC with small additive effects were detected and were significant only in one year. Three QTLs for ASS were identified; qASS-6a and qASS-6b were significant in both years, and qASS-6a corresponded to a major gene located in the alk region on chromosome 6, while qASS-3 on chromosome 3 was significant only in one year. Five QTLs for GC were detected and all were significant only in one year. The lock of detection of major gene(s) for GC may be due to the fact that none of the parents were differentiated in terms of GC. These results showed that AC and ASS were mainly controlled by known gene loci, i.e., wx and alk, respectively, with modification by minor genes.
rice,, physico-chemical property,, quantitative trait loci,, environment effect.,
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万建民, 翟虎渠**, 曹树青, 陆巍, 张荣铣**李良璧, 匡廷云, 闵绍楷, 朱德峰, 程式华
中国科学(C辑)2002,32(3):211~217,-0001,():
-1年11月30日
以杂交水稻协优63为对照,对亩产800kg以上的超高产杂交水稻组合协优9308抽穗前、后的物质生产特性及剑叶光合作用与穗部物质积累的关系进行研究。结果表明:(i)与协优63相比,协优9308抽穗前、后的物质生产能力无论生物量还是日干物质生产量,均极显著高于协优63,尤以抽穗后更为明显。(ii)协优9308剑叶的光合碳同化能力(叶源量)极显著高于协优63,且能够切合籽粒灌浆需求,在单株水平上其净同化产物基本满足籽粒灌浆的需求,而协优63抽穗后20d左右剑叶光合功能快速衰退,单株净同化产物不能满足籽粒灌浆的需求。结果证明,灌浆后期仍能保持高效光合功能、并且能够切合籽粒灌浆需求是实现水稻超高产的关键环节。
水稻,, 超高产,, 光合作用,, 籽粒灌浆
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【期刊论文】Detection and analysis of QTLs for ferrous iron toxicity tolerance in rice, Oryza sativa L.
万建民, Jian-lin Wan, Hu-qu Zhai, Jian-minWan, ∗ &H. Ikehashi
Euphytica 131: 201~206, 2003.,-0001,():
-1年11月30日
A mapping population of 96 BC1F9 lines (Backcross Inbred Lines: BILs), derived by a single-seed descent method from a backcross of Nipponbare (japonica) / Kasalath (indica) // Nipponbare, was used to detect quantitative trait loci (QTLs) for leaf bronzing index(LBI), stem dry weight(SDW), tiller number(TN) and root dry weight(RDW) under Fe2+ stress condition in rice. Two parents and 96 BILs were phenotyped for the traits by growing them in Fe2+ toxicity nutrient solution. A total of four QTLs were detected on chromosome 1 and 3, respectively, with LOD of QTLs ranging from 3.17 to 7.03. One QTL controlling LBI,SDW,TN and RDW was located at the region of C955-C885 on chromosome 1, and their contributions to whole variation were 20.5%, 36.9%, 43.9% and 38.8%, respectively. The QTL located at the region of C955-C885 on chromosome 1 may be important to ferrous iron toxicity tolerance in rice. Another QTL for SDWand RDWwas located at the region of C25-C515 on chromosome 3, with respective contributions of 47.9% and 35.0% to whole variation. Further, two QTLs on chromosome 1 were located for RDWat the region of R2329-R210 and for TN at the region of R1928-C178. Comparing with the other mapping results, the QTL located at the region of C955-C885 on chromosome 1 was identical with the results reported previously. There is a linkage between a QTL detected under Fe2+ stress condition for stem and root dry weight and a QTL detected under phosphorus-deficiency condition for dry weight on chromosome 3.
BIL population,, ferrous iron toxicity tolerance,, QTL analysis,, rice
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万建民, Jianming WAN), Seiji YANAGIHARAn), Hiroshi KATO), and Hiroshi IKEHSHI)
lapan. J Rreed 45: 507~516 (1993),-0001,():
-1年11月30日
Allelic interaction causing gamete abortion in wide crosses of cultivated rice was earber identified at locus S-5 on Chromosome 6 and recently at locus S.7 on Chromosome 7 At S-5 1ocus Indica and Japonica does have S-5i and S-5j, respectively, and Javaaicas such Ketan Nangka have a neutral allele 3-5n. The S-5i/S-5j genotype produces semi-sterile panieles due to abortion of female gametes carting S-5j, but both S-5n/S-5i and S-5n/S-5j genotypes do not. The S-5n is called "wide compatibility gene" (WCG), as one of parents possessing this allele does not show hybrid sterility in their F1. The WCG has been used for breeding hybrid varieties between Indica and Japonica rice. The S-5n allele was incorporated from Ketan Nangka to Japonica lines such as Nekken 1 or 2, which do not show semi-sterility in their cross to many Indica type. However, Nekken 2 showed typical hybrid sterility when it was crossed to a Korean Indica line. Yeong Pung. Therefore. a new Iocus was suggested for the semi-sterility in that hybrid. By genetic analyses using isozyme markers, a new locus for the hybrid stenhty between Nekken 2 and the Korean Indicas was located near Cat-I locius on Chromosome 6, and designated as following our recent report on S-7 On the basis of allelic interaction causing fenale gamete abortion, three alleles were found at S-8 follows: a Japonica line Nekken 2 and Yeong Puny possess S-8kn and S-8yp, respectively, and their hybrid genotype of S-8kn/S-8yp shows semi-sterility a portion of female gametes carrying S-8kn was aborted A Japonica tester variety, Akihikari was found to have a neutral allele, S-8n at this locus, and another neutral allele, S-7n at S-7, A possibility to cumulate three neutral alleles in a breeding line is suggested to solve hybrid sterility problem in wide crosses of rice.
Oryza sativa,, hybrid sterility,, gamete abortion,, wide compafibility,, wide cross.,
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万建民, Jianmin Wan) and Hiroshi lkehashi)
,-0001,():
-1年11月30日
The presence of semi-sterile panicles in Indica-Japonica crosses of rice is due to an allelic interaction at a gamete abortion locus, S-5 on Chromosome 6. The S-5n allele is referred to as "wide compatibility gene" (WCG), since one of the parents carrying this allele does not show hybrid sterility in the F1 llowever, Ketan Nangka, which is a donor of S-5n, showed typical hybrid sterility when it was crossed to local varieties from Tai-hu Lake, such as Fengjingdao and Feilaifeng, as well as to those from Yun-nan Province, such as Dabai, Luanjiaolong and Xiaobaigu. A new locus for the sterility in the hybrid between Ketan Nangka and the local variceties was detected near the Est-5 locus on Chromosome 1, and designated as S-16 following our recent reports on S-7, S-8, S-9 and S-15 Three alleles were detected at S-16 as follows: Ketan Nangka and the Chinese varieties carried S-16kn and S-16j, respective-ly, and the hybrid genotye of S-16kn/S-16j showed semi-sterility due to partial abortion of the female gamete car-rying S-16j. A Japonica tester variely Akihikari and an Indica tester IR 36 were found to have a neutral allele S-16n at this locus. In addition to the unique allele at S-16, the native varieties from Tai-hu and Yunnan carried S-7ni at a relatively high frequency, while among the present varieties in the continent few varieties carried the allele, as indicated by the fertility of the hybrids between them and Ketan Nangka. These unique alleles in Chinese local varieties appear to reflect their common origin and isolation from major varietal grouns.
Oryza sation,, hybrid sterility,, gamete abor-tion,, wide compatibility,, wide cross.,
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【期刊论文】Isozyme Divergence between Maintainers and Restorers in Hybrid Rice Breeding Programs in India
万建民, P. S. Devanand, J. Wan, M. Rangaswamy, and H. ikehashi*
Reprinted from Crop Science Vol 39, No.3,-0001,():
-1年11月30日
In hybrid rice breeding programs, genetic diverity between maintainers and restore, enhances heterosis. The objective was to measure the level of diversity between the two groups of rice (Oryza sativa L.) breeding lines or cultvars which were screened as potential maintainers or restnrers by test-cross to WA (wild abrtive)-cytoplasm. Allele frequencies at 15 isozyme loci were compared between 51 potential maintainers and 56 potential restorers. Differences between the two types were found at eight isozyme Iocl on chromosomes 4, 6, 7, 8,11, and 12. The majority of the potential maintaines exhibited japonica type alleles, whereas most of the potential restorers exhibited indica type alleles. This pattern is consdered to be due to retained associations between Rf (restorer)/rf (maintainer) genes and genetic markers on chromosomes other than those carrying Rflrf genes. The diversity as well as differentiation of indica and japonica type alleles between maintainers and restorers should contribute to enhanced heterosis in hybrid rice.
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【期刊论文】Identification of Marker Loci for Seed Dormancy in Rice (Oryza saliva L.)
万建民, I Wan, T. Nakazaki, * K. Kawaura, and H. Ikehashi
Reprinted from Crop Science Vol. 37. No.6,-0001,():
-1年11月30日
In rice breeding, genes for seed dormanoy as well as the markers for each of them are required of an incorporation of an adequate seed domancy into japonica cultivrs To screen genetic Ioci for seed dormancy in rice (Orvza saliva L.), two kinds of populations were tested: (i)an Fz population of a hybrid Milyang 23 I Todorokiwase. and (fi) Fl populations from a three-way cross, in which an indica donor of strong dormancy, IR 36, was crossed with non-dormant japonica cultivars, Nekken 2 and Miyukimochi, in the form of IR 36/ Nekken 2// Miyukimochi. The seeds predated on the F2 plants from the single cross and those on FI plants from the three-way cross were tested at three times: immediately after maturation, after 60 d storage at 4C, and after breaking dormancy (7 d at 50C). Of 24 marker Ioci assayed, at least fore loci linked to seed dormancy were detected. The strong seed dormancy of Milyang 23 was linked with three isozyme genes: Pgi-I1 on chrompsome 3, Amp-32 on chromosome 6, and Est92 on chrmosome 7, while the strong seed dormancy of IR 36 was linked with C+ (apiculus color) on chromosome 6, Est-92 on Chromosome 7, and Acp-21 an chromosome 12. The high level of seed dorloci. Differential responses of inch Iocl to a dormacy-breaking treatment were detected. An allele from IR 36 at a locus on chromosome 6 seemed to be responsible for strong seed dormany after maturation and to be easliy broken during storage. This allele may be incorporated into japonica ries by means of marker C for apiculus color.
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【期刊论文】Two new loci for hybrid sterility in cultivated rice (Oryza sativa L.)
万建民, J. Wan. Y. Yamaguehi
Theor Appl Genet (1996) 92: 183~190,-0001,():
-1年11月30日
Female gamete abortion in Indica-Japonica crosses of rice was earlier identified to be due to an allelic interaction at the S-5 locus on chromosome 6. Recently. in other crosses of rice, similar afielic interactions were found at loci designated as S-7 and S-8, located on chromosomes 7 and 6 respectively. All of them are independent of each other. At the S-5 locus, Indica and Japonica rice have S-5i and S-Y alleles respectively and Javanieas, such as Ketan Nangka, have a neutral allele S-5". The S-5i/S-5j genotype is senti-sterile due to partial abortion of female gametes carrying S-U, hul both the S 5"/S-5i and S-5"/S-5J genotypes are fertile. The S-5" allele is thus a "wide-compatibility gene" (WCG), and parents homozygous for this allele are called wide-com patible varieties (WCV), Such parents when crossed with Indica or Japonica varieties do not show F1 hybrid sterility. Wide compatible parents have been used to overcome sterility barriers in crosses between Indica and Japonica rice, However, a Javanica variety, Ketan Nangka (WCV), showed typical hybrid sterility when crossed to the Indian varieties N22 and Jaya. Further. Dular+ another WCV from India, showed typical hybrid sterility when crossed to an I RRI line, IR2061-628 1-6-4-3(IR2061-628). By genetic analyses using isozyme markers, a new locus causing hybrid stetihty in crosses between Ketan Nangka and the Indicas was located near isozyme loci Est-I and Mal-I on chromosome 4, and w as designated as S-9. An other new locus for hybrid sterility in the crosses between Du]ar and the IR2061 628 was identified and was found lin ked to four isozyme loci, Sdh-l, Pox-g, Aep-1 and Acp-2. on chromosome 12 It was designated as S-15. On the basis of allelic interac tlons causing female-gamete abortion, two alleles were found at S-9, S-9kn in Ketan Nangka and S-9iin N22 and Jaya. In the heterozygote, S-9kn/S-9i, which was semisterile, female gametes carrying S-9kn" were aborted. The hybrid of Dular and IR2061-628, with a genetic constitution of S-15Du/S-I5i, was semi-sterile and the female gametes carrying S-I 5Du were aborted. A Japonica tester variety, Akihikari, and an Indica variety, IR36, were found to have neutral alleles, S-9n and S-15n, at these loci, in addition to S-7" and at S-7. The accumulation of three neutral alleles into a breeding line should help solve the hybrid sterility problem in wide crosses office.
Oryza sativa
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